Re[4]: [DIYbio] Substitute for calf serum?

11:57 PM |

So far my understanding is that for culturing stem cells the medium consists of  a base containing nutrients and antibiotics such as DMEM/F12. Then typically 20% FBS is added for the growth factors. This is the part that is now commonly substituted by more precise or human derived component. Platelet lysate seems to be the recommended substitute.  Since one of the goals of this experiment is to make it as cheap as possible, I would like to make my own platelet lysate  rather than buy it (https://www.stemcell.com/human-platelet-lysate.html). I've made PRP and PRF many times. What i don't know how to do is figure out how much to make and add to the base medium.

------ Original Message ------
From: "Bryan Jones" <bryanjjones@gmail.com>
Sent: 4/14/2021 11:44:07 AM
Subject: Re: Re[2]: [DIYbio] Substitute for calf serum?

Here's a pretty good summary article on different alternatives to FBS. https://pharma.lonza.com/-/media/Lonza/knowledge/CGT/A_consensus_introduction_to_serum_replacements_CGT.pdf
There is also a database of articles that have reported non-FBS growth media for various cell types: https://fcs-free.org/fcs-database
I think what S James Parsons is talking about is a defined media (where each protein, growth-factor, salt, and other component is explicitly defined) . There are companies that make defined media, but I'm not sure if any are as good or affordable as FBS-based media. It is very desirable to use something defined and animal-free for any GMP product or product to be used in the clinic.
--Bryan Jones


On Mon, Apr 12, 2021 at 1:20 PM Frank <fgarcia0007@gmail.com> wrote:
I did a little more reading https://www.frontiersin.org/articles/10.3389/fcell.2016.00115/full and it turns out that human serum works even better than calf serum. derivatives of this that are  serum, plasma, platelet rich plasma, platelet poor plasma, and platelet lysate. I can easily make these myself from my own blood for the price of blood collection tubes or $0 with zero cruelty compared to $1000 for a liter of FBS which is obtained in a hideously cruel way. This article and others ouitline the many advantages to abandoning calf serum.

Yay!


------ Original Message ------
From: "S James Parsons Jr" <sjamesparsonsjr@gmail.com>
Sent: 4/12/2021 10:09:31 AM
Subject: Re: [DIYbio] Substitute for calf serum?

Human serum would work, the problem is cost and amount of material required.  

My idea is to create a profile of FBS, salt, proteins, the the like. Then find the minimum of each protein, salt, and growth factor needed for tissue culturing.   Once identified, create a transmutated organism (yeast, bacteria) to produce said proteins.  Then use that elixir as a replacement for FBS.

Boom, free hamburgers for life! 




On Apr 12, 2021, at 12:33 AM, Frank <fgarcia0007@gmail.com> wrote:

Thanks James or responding to my question. Your answer made me curious as I find it hard to believe that there could only be one product on the market for anything .  I found what appears to be a bunch of products marketed as substitutes for calf serum with some interesting advantages. Here are a couple. What do you think of these? Are they calf serum alternatives

Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media.  C Rauch, E Feifel, EM Amann, HP Spötl… - ALTEX-Alternatives to …, 2011 - altex.org

 

------ Original Message ------
From: "S James Parsons Jr" <sjamesparsonsjr@gmail.com>
Sent: 4/11/2021 3:07:16 PM
Subject: Re: [DIYbio] Substitute for calf serum?

That is the holy grail of tissue culturing.  If you find a magic elixir with growth hormones, salts, proteins, enzymes, and a desired pH you would be a wealthy person! 

On Apr 11, 2021, at 2:52 PM, Frank <fgarcia0007@gmail.com> wrote:

Can anyone please tell me if I can use a substitute when a cell culturing protocol calls for calf serum? specifically, a substitute that does not involve cruelty to animals. is it possible to use my own blood serum?


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