Re: [DIYbio] Re: Geneome Sequencing Costs

> Separate molecules in the same lane, or ligated end-on-end? Wouldn't
> parallel reactions lead to multiple peaks simultaneously, leading to
> difficulty establishing which molecule has which nucleotide?*

Illumina HiSeq can handle millions of reads per lane. The problem
isn't separating the peaks, but rather differentiating which read came
from which sample, assuming you merged multiple samples together. The
solution is to ligate a different short sequence on to the end of each
sample. So, for example, sample 1 gets ATGGCA ligated on the end and
sample 2 gets GCCTAA. That way you can separate the reads out later.

-cory

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