You should be able to grow many cells in modified media very similar to what HeLa or HEK cells grow in. Just google for protocols some cells have slightly different requirements.
The people discouraging you to work with cells because you might catch a disease are just paranoid and/or have never worked with human cell lines before.
Ask 99% of Scientists why they do cell culture in negative pressure flow hoods and containment and they will say because they don't want the culture to become infected with fungus or bacteria not because they are afraid of catching a disease.
1. Who uses a needle for cell culture?
2. Has there ever been a documented case of someone acquiring a disease from cell culture?
3. You swallow your own cells and other people's cells all the time.
Cathal that is ridiculous. I have worked with cell culture a ton(HEK, HeLa, breast cancer cell lines) and have never had to be vaccinated against anything for that purpose. No one I know has been. EU laws maybe at fault?
On Saturday, June 22, 2013 3:44:37 PM UTC-5, Mega wrote:
Hi everyone,--
I may have to get a wisdom tooth removed someday...
I remember it was said that there are stem cells in the pulpa / or in a gum pocket.
From what I read from google, it doesn't seem to be stem cells, but cells that can be induced pluripotent stem cells with high efficiency... Other articles sound like there actually are stem cells ready-to use. One article said it is adult stem cells, which are somewhat differentiated, so they can only form bones and certain other tissues...
Has anyone read about that?
If they actually are adult stem cells, can I take them, desinfect them and put them into Hela/A431/CHO medium and grow them? Without induction they may not grow into a tooth again, but maybe they divide?
Best,
Andreas
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