I remember a paper one/two years ago from William C. Earnshaw group which succeed in making a HAC (Human Artificial Chromosome) to pass through a few mouse generations. I don't think it has ever been used in human though but yeah stability is one of the major problem (also to be sure the HAC doesn't interact with other chromosomes).
-- Currently it seems CRISPR is what is used for germline editing both in different species. In human, George Church lab has made a try but the paper is not yet published and there are rumours of success by Chinese groups but until the publications came out we don't really know...
The idea for using CRISPR is to edit the hESC and then to develop them in sperm or gametes which is far more easier to perform than modifying the embryo directly (hESC is easy to obtain and grow whereas embryo is far more difficult to obtain and there is not a lot available).
The best country for doing this kind of experiments right now must be China since they don't seem to have any problem in performing it. In USA some scientists have already called for a moratorium but we still don't know if it will be followed.
On Friday, 27 March 2015 23:10:22 UTC, Koeng wrote:
On Friday, 27 March 2015 23:10:22 UTC, Koeng wrote:
New YACs are known to be highly unstable, which are actually why they started using BACs instead during the human genome project. I'd assume it'd be the same for a new human one.Possibly use a viral system to excise the DNA in the presence of, lets say, lactose and tetracycline, but only when in germ line cells.
On Friday, March 27, 2015 at 3:19:46 PM UTC-7, Nathan McCorkle wrote:I've got an idea that you could make a condom-catheter type
microfluidic. I've read about male/female sorting of sperm, but how do
you edit the DNA? I know about CRISPR, transposons, and probably some
other similar things I can't remember, but any other ways that have
been thought of or experimented with?
Is adding a new chromosome a better idea? Would this prevent the 'new'
chromosome from being passed during future breeding with a
non-modified person? (I think that would make the neo-human less
offensive to anti-GMO folks)
Previously I've asked what political/social climate would be most
conducive to this work, and I think the answer was 'south korea', but
that was a few years ago, and I wonder if there's a better place these
days. Not necessarily a country with active research, but one that
would be supportive of active evolution, rather than being fearful?
--
-Nathan
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