http://www.pnas.org/content/103/47/17672.full
Here is a protocol how to do it. Basically, sperm has a layer that protects it from foreign DNA (alledgedly) so you purify it, mix it with DNA and do artificial/instrumental insemination of a female specimen. This episome is said to be totally stable, and is replicated during mitosis (almost as stable as a chromosome). It has an element that attaches to the chromosomes during mitosis and is co-replicated. It does not integrate so no chance of getting cancer from random integration and hetereochromatin unwinding/activation of downstream genes.
There also is an improved version of the plasmid with a native human promoter (gets less silenced).
-- Here is a protocol how to do it. Basically, sperm has a layer that protects it from foreign DNA (alledgedly) so you purify it, mix it with DNA and do artificial/instrumental insemination of a female specimen. This episome is said to be totally stable, and is replicated during mitosis (almost as stable as a chromosome). It has an element that attaches to the chromosomes during mitosis and is co-replicated. It does not integrate so no chance of getting cancer from random integration and hetereochromatin unwinding/activation of downstream genes.
There also is an improved version of the plasmid with a native human promoter (gets less silenced).
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/6c4e28bb-067e-4702-9730-cf0ca95c6c11%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.
0 comments:
Post a Comment