You might want to have something that increases expression when it is cold http://www.nature.com/scitable/topicpage/environmental-influences-on-gene-expression-536 some genes in rabbits are activated by lower temperature. Although if the protein doesn't damage the plant/not a health concern it should be fine to have constant expression. The other issue is finding a protein that protects plant tissues from frost damage and getting those gene(s) into the plant.
On Sunday, August 9, 2015 at 8:42:40 PM UTC+9, Cathal Garvey wrote:
-- On Sunday, August 9, 2015 at 8:42:40 PM UTC+9, Cathal Garvey wrote:
I think the main issue with cold isn't that enzymes slow down, but that
frost causes structural damage to the plant. So, the fish proteins used
for other cold-resistant crops weren't chaperones exactly, they were
antifreeze proteins that prevent ice crystals from forming.
And, they work, although because they bind ice crystals directly I
imagine they work best if they are expressed in proportion to ice hazard?
On 09/08/15 11:56, Mega [Andreas Stuermer] wrote:
> Hi, it is a great idea, but do you know where the bottleneck is? Are all
> its proteins optimized for higher temperatures? Then it will be pretty
> difficult. Only one or few proteins - doable. Unless you add some exotic
> arctic ( was it shrimp?) chaperons that keep all the enzymes working at
> lower temperatures.
>
>
>
>
>
>
> On Saturday, August 8, 2015 at 7:43:51 PM UTC+2, John D wrote:
>
> Hello Fellow Biohackers,
>
> I want to work on creating cold resistant forms of wheat. I don't
> have a lot of experience working with plants; mostly I have done
> ecology research. I have done some work in phylogenetics as well. If
> anyone has any resources, learning materials, etc, or know of any
> labs in the Boston area that I could work with that would be wonderful!
>
> Best,
> John
>
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