Re: [DIYbio] Re: Want to work on creating cold resistant wheat

If it is "just" about expressing antifreeze protein in wheat, that seems fairly doable. Probably you also find a cold induced promoter out of the literature.

I was told that monocots are different to regenerate from a gew transformed cells, though.
Agrobacterium may be easier, but then your organism is regulated by FDA and USDA.


Here is a small intro to plant trafos (transient). Might be worth a look.
http://diyspartanbiotech.files.wordpress.com/2014/03/genetic-engineering-and-synbio-for-beginners-v1.pdf

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