Probably want to include a reporter gene such as a gfp so you don't have to sequence all of the plants.
On Sunday, August 9, 2015 at 9:22:29 PM UTC+9, Mega [Andreas Stuermer] wrote:
-- On Sunday, August 9, 2015 at 9:22:29 PM UTC+9, Mega [Andreas Stuermer] wrote:
If it is "just" about expressing antifreeze protein in wheat, that seems fairly doable. Probably you also find a cold induced promoter out of the literature.I was told that monocots are different to regenerate from a gew transformed cells, though.
Agrobacterium may be easier, but then your organism is regulated by FDA and USDA.
Here is a small intro to plant trafos (transient). Might be worth a look.
http://diyspartanbiotech.files.wordpress.com/2014/03/ genetic-engineering-and- synbio-for-beginners-v1.pdf
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