Ok....not sure about all this since I believe the devil is always in the details, but I have no expertise here and won't pass judgment.
My only comment is once you have your cDNA, make sure you alter the sequence to prioritize synonymous codons favored for expression in E. Coli or whatever you are inserting this into. I don't know how far of codon usage bias or even the genetic code is between SARS-CoV-2 and E. Coli but I suspect there are important differences.
On Sunday, April 5, 2020 at 7:02:06 PM UTC-4, Zonie deep wrote:
You know if we are desperate enough it would be quite easy to make your own purified recombinant covid spike protein in bacteria. This could be used for a vaccine without too much regulatory. Protein could be made in a few weeks. Need to be sterile of course, dosage worked out. I've only immunized rabbits with these types of preps. Always get good antibodies,no harm to rabbits.
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