Never had a problem with codon usage. Bacteriophage love E. coli. Many large proteins degraded when expressed in bacteria but small pieces like this usually quite stable and highly expressed. Especially when made as fusion proteins. To express like this is routine. Many companies have made spike protein already.
On Tue, Apr 7, 2020, 8:58 AM Reginald Smith <rsmith@supremevinegar.com> wrote:
--Ok....not sure about all this since I believe the devil is always in the details, but I have no expertise here and won't pass judgment.My only comment is once you have your cDNA, make sure you alter the sequence to prioritize synonymous codons favored for expression in E. Coli or whatever you are inserting this into. I don't know how far of codon usage bias or even the genetic code is between SARS-CoV-2 and E. Coli but I suspect there are important differences.
On Sunday, April 5, 2020 at 7:02:06 PM UTC-4, Zonie deep wrote:You know if we are desperate enough it would be quite easy to make your own purified recombinant covid spike protein in bacteria. This could be used for a vaccine without too much regulatory. Protein could be made in a few weeks. Need to be sterile of course, dosage worked out. I've only immunized rabbits with these types of preps. Always get good antibodies,no harm to rabbits.
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/e15f76aa-1c4c-407a-9568-b8f544fb6b33%40googlegroups.com.
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CAFrs6Pin-_nUYedt8cOHk-YPf_pW_K9zDfp5gUCSrxENQmqUpg%40mail.gmail.com.
0 comments:
Post a Comment