Do you think they will send me some seeds of that plant ;) ?
No, honestly, will they?
On 13 Nov., 03:55, Idan Efroni <ida...@gmail.com> wrote:
> The first thing you need to know that it has been done, at least in
> tobbaco.
> See here:http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.00...
> (open access)
>
> The basic idea was somewhat as outlined here - get the lux operon, add
> flanking regions of chloroplast DNA, shoot the DNA in, and select for
> positives. While one can think of different ways to do it, this is
> certainly the simplest. It does require a) a gun, b) the sequence of
> the plant chloroplast DNA.
>
> Given this, I don't see a reason why this shouldn't work for any other
> plant. As you may have already understood, transforming plants is
> somewhat of an art, and if you want to venture away from the commonly
> used ones, there is going to be a lot of trial and error. The
> particular hormones and conditions of regeneration can vary to a large
> extent, and some just won't regenerate (God knows I tried).
>
> Lichens is an interesting but not so well investigated system.
> Assuming it is a cyano-fungi (and not algea-fungi) lichen, it might
> work. You will probably need a plasmid which is compatible with cyano,
> as the e.coli ones are unlikely to work. There are plasmids out there,
> but this means sub-cloning. Selection is bound be pretty hard on such
> a slow growing organism, though.
>
> How about green algea? There is bound to be some chlamydomonas
> swimming happily in a pond in a park near you. They should be PEG
> transformable. My guess is that the e.coli plasmid by itself wouldn't
> replicate well in the chlamy chloroplast, so your best bet is to aim
> for chromosome integration as in the plos one paper.
>
> Idan
>
> On Nov 7, 4:11 am, Mega <masterstorm...@gmail.com> wrote:
>
> > Ok, what I learned so far...
>
> > Firstl, it's no big deal for a pro to insert a lux operon containing
> > plasmide in an e.coli cell to make it glow. the antibioticum
> > ressistance and lacZ' will make it selectable.
> > Usuall, there's no more quorum sensing because the have cut it out.
>
> > - ''Just'' insert the plasmide into a bacterium and it glows.
>
> > To engineer a plant on the other hand is a much more difficult issue.
>
> > But what about a path in the middle (don't know if this term is used
> > in english ;) )?
>
> > Lichens consist of a fungus and canobacterium.
>
> > So, i take a plasmide that contains lux and ampecillin resistance.
>
> > Now i transform very small pieces of the lichen. Some of the
> > Cyanobacteria will be transformed and ressistant to ampecilline.
>
> > The fungus should either be ressistant to ampecilline on its own
> > (true??) or the cyano spread the ampecilline-degenerating substance
> > within the whole lichen.
>
> > Polyethleneglycol will help the endoctose to take place...
>
> > Then I'll grow them on a Lamp plate to select the lichens that include
> > ressistance. Of the bacteria inside the lichen, the ones containing
> > the most ampecilline-degenerating substances, these will grow fastest
> > and so they will mitose much more often.
>
> > I think that could be a way to go?
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