Not a hope in hell, I'm afraid!
That said, it's awesome to see that it worked. And that's with (AFAIK
after a quick skim of the paper) with a basically unoptimised operon;
you could seriously improve that bioluminescence with a little work. As
I mentioned previously, there's lots of work out there in improving
light production by de-coupling transcription of LuxAB from the rest,
and you could probably gain another factorial leap in brightness if you
somehow upregulated fatty acid synthesis in the target cells/organelles.
On 14/11/11 17:52, Mega wrote:
> Do you think they will send me some seeds of that plant ;) ?
> No, honestly, will they?
>
> On 13 Nov., 03:55, Idan Efroni <ida...@gmail.com> wrote:
>> The first thing you need to know that it has been done, at least in
>> tobbaco.
>> See here:http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.00...
>> (open access)
>>
>> The basic idea was somewhat as outlined here - get the lux operon, add
>> flanking regions of chloroplast DNA, shoot the DNA in, and select for
>> positives. While one can think of different ways to do it, this is
>> certainly the simplest. It does require a) a gun, b) the sequence of
>> the plant chloroplast DNA.
>>
>> Given this, I don't see a reason why this shouldn't work for any other
>> plant. As you may have already understood, transforming plants is
>> somewhat of an art, and if you want to venture away from the commonly
>> used ones, there is going to be a lot of trial and error. The
>> particular hormones and conditions of regeneration can vary to a large
>> extent, and some just won't regenerate (God knows I tried).
>>
>> Lichens is an interesting but not so well investigated system.
>> Assuming it is a cyano-fungi (and not algea-fungi) lichen, it might
>> work. You will probably need a plasmid which is compatible with cyano,
>> as the e.coli ones are unlikely to work. There are plasmids out there,
>> but this means sub-cloning. Selection is bound be pretty hard on such
>> a slow growing organism, though.
>>
>> How about green algea? There is bound to be some chlamydomonas
>> swimming happily in a pond in a park near you. They should be PEG
>> transformable. My guess is that the e.coli plasmid by itself wouldn't
>> replicate well in the chlamy chloroplast, so your best bet is to aim
>> for chromosome integration as in the plos one paper.
>>
>> Idan
>>
>> On Nov 7, 4:11 am, Mega <masterstorm...@gmail.com> wrote:
>>
>>> Ok, what I learned so far...
>>
>>> Firstl, it's no big deal for a pro to insert a lux operon containing
>>> plasmide in an e.coli cell to make it glow. the antibioticum
>>> ressistance and lacZ' will make it selectable.
>>> Usuall, there's no more quorum sensing because the have cut it out.
>>
>>> - ''Just'' insert the plasmide into a bacterium and it glows.
>>
>>> To engineer a plant on the other hand is a much more difficult issue.
>>
>>> But what about a path in the middle (don't know if this term is used
>>> in english ;) )?
>>
>>> Lichens consist of a fungus and canobacterium.
>>
>>> So, i take a plasmide that contains lux and ampecillin resistance.
>>
>>> Now i transform very small pieces of the lichen. Some of the
>>> Cyanobacteria will be transformed and ressistant to ampecilline.
>>
>>> The fungus should either be ressistant to ampecilline on its own
>>> (true??) or the cyano spread the ampecilline-degenerating substance
>>> within the whole lichen.
>>
>>> Polyethleneglycol will help the endoctose to take place...
>>
>>> Then I'll grow them on a Lamp plate to select the lichens that include
>>> ressistance. Of the bacteria inside the lichen, the ones containing
>>> the most ampecilline-degenerating substances, these will grow fastest
>>> and so they will mitose much more often.
>>
>>> I think that could be a way to go?
>
--
www.indiebiotech.com
twitter.com/onetruecathal
joindiaspora.com/u/cathalgarvey
PGP Public Key: http://bit.ly/CathalGKey
--
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio?hl=en.
0 comments:
Post a Comment