On 11/15/2011 08:24 AM, Cathal Garvey wrote:
> Of course, a kickstarter could probably fund synthesis, which would be
> nice for a few reasons: The enzymes are usually prepared industrially
> with chromatography (HPLC), which is hard to reproduce.
Do you mean expensive to operate HPLC, or technically difficult to get results the same?
You'd waste a
> lot of time cloning the gene into a form that's easy to isolate
Sounds like a job for a it manufacturer.
> (ice-purification is nice, but I like the look of elastin-like-polymers
> for a DIY-friendly enzyme prep), and spend a lot of money on appropriate
> enzymes, primers and buffers.
What does elastin do?
John
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