I'm not as pessimistic about reagent preps; with the right biotech
behind the enzymes, preparing and purifying them should be as easy as
putting on an overnight culture, and performing a standardised
extraction the next day, like a miniprep for plasmid DNA.
That said, I'd *happily* buy "Genspace's Finest EcoRV", or "DIYbioMCR's
Old-timer PfuBru".
On 15/11/11 14:43, jlund256 wrote:
>> pretty well suited for this. Another potential money saver would be a
>> publicly available set of plasmids easily digested into quality DNA
>> ladders. $50 per tube of restriction enzyme and $1 per lane of commercial
>
> I think it is a great idea. DNA ladders can be fairly easy to make.
> The original ones were lambda phage DNA digested with HindIII or
> HindIII/EcoRI. Labs had lots of lambda phage so ladders were easy to
> make.
>
> While most biological reagents are (or can be) fairly easy to make,
> production typically requires several days per reagent. An
> independent DIY lab would spend nearly all its time making reagents.
> So shared reagent labs or larger reagent exchanges are going to be
> necessary. A dozen labs each making two or three restriction enzymes
> in 100ml (100,000 activity unit) batches could keep the entire
> community supplied. A model where each lab makes a few reagents for
> community use and draws on other labs for their other needs seems the
> way to go. The place for individuals to start is to begin making a
> community reagent and offering it around.
>
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