> pretty well suited for this. Another potential money saver would be a
> publicly available set of plasmids easily digested into quality DNA
> ladders. $50 per tube of restriction enzyme and $1 per lane of commercial
I think it is a great idea. DNA ladders can be fairly easy to make.
The original ones were lambda phage DNA digested with HindIII or
HindIII/EcoRI. Labs had lots of lambda phage so ladders were easy to
make.
While most biological reagents are (or can be) fairly easy to make,
production typically requires several days per reagent. An
independent DIY lab would spend nearly all its time making reagents.
So shared reagent labs or larger reagent exchanges are going to be
necessary. A dozen labs each making two or three restriction enzymes
in 100ml (100,000 activity unit) batches could keep the entire
community supplied. A model where each lab makes a few reagents for
community use and draws on other labs for their other needs seems the
way to go. The place for individuals to start is to begin making a
community reagent and offering it around.
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