On Wednesday, 4 April 2012 14:31:25 UTC+8, Nathan McCorkle wrote:
Using the old googlulator, I found a paper transforming lactobacillus
acidophilus NCFM via homologous recombination using no viruses which
showed integration with as little as 169bp of homology on either end
of the insert, and stability over 35 generations. They used 500ng
plasmid DNA (from their cloning operations) 12.5kV/cm electroporation
and some special buffers... which could probably be done without/DIYedhttp://www.scribd.com/doc/
87924827/Appl-Environ- Microbiol-2011-Douglas-7365-71
I am not sure, but doesn't the upp-counterselectable gene replacement system requires a plasmid? Or can we just insert a linear construct?
Thanks!
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