This seems decent, seller says it puts out 9.5kV, for $15
http://cgi.ebay.com/ws/eBayISAPI.dll?ViewItem&item=180855665615&ssPageName=ADME:X:RTQ:US:1123#ht_620wt_1144
I bet you could do pretty good with a charge pump to bump this up to
100kV... if you can get components rated for ESD higher than that.
I don't know if 100kV at whatever current that would be, would be
enough to do a 60cm capillary gel electrophoresis though.
On Fri, Apr 6, 2012 at 9:56 PM, Jonathan Cline <jncline@gmail.com> wrote:
>
>
> On Friday, April 6, 2012 12:00:06 AM UTC-7, Cathal wrote:
>>
>> A friend of mine simply uses rectified wall outlet voltage. He doesn't
>> even bother with a capacitor to smooth it, so it's really variable.
>> Apparently it works fine..
>
>
>
> Rectifying the wall AC works fine, until you kill yourself. That's about as
> safe as using a hairdryer while sitting next to a full bathtub -- think
> about it. The wall outlet can source 30 amps before some breaker trips.
> Quite enough to turn organs into cooked hot dogs. Sure, your friend might
> say that all wires are protected, has fuses, etc, etc, it's still not a
> great idea without a real current limiting circuit internal to the equip.
> Perhaps he's using a big isolation transformer, that might be OK depending
> on placement of fuses in the circuit, but the supply is likely pretty large
> & heavy compared to a solid state version.
>
> So: Don't do electrophoresis with wall AC directly.
>
> There are several papers which study the effect of ripple on
> electrophoresis, they all reach the same conclusion: ripple doesn't matter
> (it just slows down the run, slightly, to the same as that of the equivalent
> voltage).
>
> As for comparing wall AC to CCFL HVPS output, the voltage of the wall is
> 220V max (in EU), whereas CCFL HVPS may be much higher (depends on the
> supply), I got a couple in 400-600V range (depends on load).
>
>
> ## Jonathan Cline
> ## jcline@ieee.org
> ## Mobile: +1-805-617-0223
> ########################
>
>
>
>>
>> Jonathan Cline <jncline@gmail.com> wrote:
>>
>> >
>> >
>> >On Thursday, March 22, 2012 7:01:28 AM UTC-7, Nathan McCorkle wrote:
>> >>
>> >> At $10/gel the pre-cast option still sucks.
>> >> http://products.invitrogen.com/ivgn/product/G521801
>> >>
>> >> I really just want a solid HV power supply... Voltage and current
>> >> control with banana plugs.
>> >>
>> >
>> >At those dimensions, I believe a high voltage power supply designed for
>> >a
>> >CCFL laptop backlight would do. (For previous generation of laptops,
>> >anyway.) They are cheap from second sources, $20-$40 range. Make
>> >sure to
>> >get one with clear pinouts though.
>> >
>> >Most high voltage power supplies designed for scientific use are low
>> >ripple, designed to be stable for traditional lab use. However DNA
>> >doesn't care if the voltage is rippling +/- 10%, the gel runs fine
>> >anyway.
>> >
>> >
>> >## Jonathan Cline
>> >## jcline@ieee.org
>> >## Mobile: +1-805-617-0223
>> >########################
>> >
>> >--
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Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics
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