Hi there!
I dunno if this has been said before, but getting foreign DNA into chromosomes depends mostly on what organism/cell line you have.
In mice or certain chicken cell lines targeted genomic integration is possible, but it is a bit time consuming. In humans this is very difficult, although there are certain cell lines (like 293) who readily integrate linear pieces of DNA randomly into themselves. In bacteria I only know E. coli, which loves to take up plasmids, although it will not actually integrate it into its genome, but it will carry it stably as an episome.
Maybe if you pick an organism what interests you the most, then we can come up with something more detailed.
Cheers,
Mat
On Tuesday, 1 May 2012 20:35:49 UTC+2, Mega wrote:
Hey guys,--
I've learned very much of the function of DNA and plasmids here...
What I yet don't know:
What are the methods to get DNA into the genome?
Could you give me some keywords so that I can do a google research on that?
(I know there are e.g. Agrobacterium transformation and bacteriophage, but what about gene gun etc. ? )
Am I right, that you can insert DNA of interest into a short gene sequence of the host, and then put it into the host?
So it will make homologuos recombination with the strand and the insert is in the genome??
Thanks!
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/45mGP1BBP-4J.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio?hl=en.
0 comments:
Post a Comment