[DIYbio] Magnetofection, Magnetophoresis, was Re: Building a particle bombardement device DIY

I guess he also uses the term magnetophoresis...

This paper seems the best:
Magnetic nanoparticles as gene delivery agents: enhanced transfection
in the presence of oscillating magnet arrays
http://diyhpl.us/~nmz787/Magnetic%20nanoparticles%20as%20gene%20delivery%20agents--%20enhanced%20transfection%20in%20the%20presence%20of%20oscillating%20magnet%20arrays.pdf

and some other decent looking papers/theses
http://edoc.ub.uni-muenchen.de/5369/1/Scherer_Franz.pdf
http://repository.library.georgetown.edu/bitstream/handle/10822/553205/spinella-mamoVincent.pdf?sequence=1

Advances in magnetofection—magnetically guided nucleic acid delivery
http://magneticliquid.narod.ru/autority/433.pdf

Insights into the mechanism of magnetofection using MNPs-PEI/pDNA/free
PEI magnetofectins
http://med-nano.sjtu.edu.cn/blog/hcgu/files/2011/10/Insights-into-the-mechanism-of-magnetofection-using.pdf

Ex Vivo Magnetofection With Magnetic Nanoparticles: A Novel Platform
for Nonviral Tissue Engineering
http://ntur.lib.ntu.edu.tw/bitstream/246246/164612/1/142.pdf

Non-Viral Gene Therapy Vectors Carrying Genomic Constructs
http://cdn.intechweb.org/pdfs/21814.pdf

this one, if for nothing but ref 1:
Towards oriented assembly of proteins onto magnetic nanoparticles
http://www.bioe.umd.edu/~kofinas/paperspdf/chiwei-bentley-biochemengjournal.pdf

this German company is selling kits, they even have tested it on cat
cells, who wanted a glowing GFP cat again?
http://www.chemicell.com/products/magnetofection/docs/magnetofection_2_4.pdf

an Indian paper using the above company's kit
New Device for Enhancement of Liposomal Magnetofection Efficiency of
Cancer Cells
http://www.waset.org/journals/waset/v58/v58-65.pdf


On Tue, Aug 28, 2012 at 5:57 PM, Nathan McCorkle <nmz787@gmail.com> wrote:
> Its a magnetic field, not electric. The magnetic particles with DNA
> bound are suspended evenly in the cell, and you apply a magnetic pulse
> in one direction (I guess a changing field could work too). Think
> magetic bead DNA separation kits, where you just place a magnet on the
> side of a microfuge tube, now add some unexpecting cells to get in the
> way, and quickly bring the strong magnet to the side of the tube...
> some magnetic beads will rip through a cell all the way to make it to
> the tube edge, some magnetic particles will get stuck mid-way through
> the cell.
>
> I heard a guy give a talk on microfluidics who used to work at Kodak,
> and now is at SUNY Buffalo, he has done some of this magnetofection
> stuff
> http://www.cbe.buffalo.edu/people/full_time/e_furlani.php
>
> On Tue, Aug 28, 2012 at 5:23 PM, Cathal Garvey <cathalgarvey@gmail.com> wrote:
>> I suppose if one were to apply a rapid AC field to the cells, it could
>> "shake" the nanoparticles through the membrane, but that field would
>> probably have to be strong enough that it's hard to distinguish it from
>> regular chemically assisted electroporation.
>>
>> Still, I've never tried it. Maybe the efficiencies are indeed crazy high
>> and toxicities low, as it reads in the article.
>>
>> On 28/08/12 19:39, Nathan McCorkle wrote:
>>> On Tue, Aug 28, 2012 at 2:33 PM, Cathal Garvey <cathalgarvey@gmail.com> wrote:
>>>> Magnetofection's article reads like an advert.
>>>
>>> hmm, i don't see it that way
>>>
>>>>
>>>> Also, if the article is to be believed, it sounds like it's more or less
>>>> 100% based on chemical transformation, with the "magneto" part only
>>>
>>> I also don't see it that way... " Their association with the gene
>>> vectors (DNA, siRNA, ODN, virus, etc.) is achieved by salt-induced
>>> colloidal aggregation and electrostatic interaction" then you apply an
>>> electromagnetic field and the magnet:DNA complex rips into the cell.
>>>
>>> More like electroporation or gene gun to me than chemical methods
>>>
>>>> describing the means of concentrating the transforming material close to
>>>> the cells. The particles are otherwise just DNA coated in "proprietary
>>>> formulations", probably some blend of PEG and molecules with some
>>>> affinity for cell surface antigens.
>>>>
>>>> On 28/08/12 18:52, Nathan McCorkle wrote:
>>>>> you could also try magnetofection or magnetoporation... some term like
>>>>> that...
>>>>>
>>>>> http://en.wikipedia.org/wiki/Magnetofection
>>>>>
>>>>>
>>>>> On Aug 27, 2012 2:35 PM, "Patrik D'haeseleer" <patrikd@gmail.com> wrote:
>>>>>
>>>>>> That's a great find!
>>>>>>
>>>>>> I think we'll just have to experiment a bit to find the right particle
>>>>>> composition. Obviously, you want something that is relatively inert,
>>>>>> preferably high density, and very small particle size. So for a
>>>>>> professional lab, gold or tungsten nanoparticles are an obvious choice.
>>>>>> That does *not* mean this is the only way to get it to work though!
>>>>>>
>>>>>> I brought up magnetite particles before, because I'd been looking into how
>>>>>> to make ferrofluid lately. It turns out there's a simple chemical route to
>>>>>> make magnetite nanoparticles that are way smaller than the magnetite powder
>>>>>> I had bought online in initially. And magnetite may not be as heavy as
>>>>>> gold, but I can tell you that jar of magnetite has some serious heft to it.
>>>>>> Probably worth a try. Plus, the magnetic nature of the particles may allow
>>>>>> us to manipulate them in ways you can't do with gold.
>>>>>>
>>>>>> Patrik
>>>>>>
>>>>>> On Monday, August 27, 2012 8:38:46 AM UTC-7, Mega wrote:
>>>>>>>
>>>>>>> I was thinking in investing into http://www.alibaba.com/**
>>>>>>> product-gs/226547546/Portable_**Gene_Gun.html<http://www.alibaba.com/product-gs/226547546/Portable_Gene_Gun.html>this one. 300$ for one device.
>>>>>>>
>>>>>>>
>>>>>>> But if the particles are this expensive...
>>>>>>>
>>>>>>>
>>>>>>>
>>>>>>>
>>>>>>>
>>>>>>> On Mon, Aug 27, 2012 at 12:22 PM, Patrik D'haeseleer <pat...@gmail.com>wrote:
>>>>>>>
>>>>>>>> Here's some more useful DIY info:
>>>>>>>>
>>>>>>>> http://thalamus.wustl.edu/**nonetlab/ResourcesF/genegun/**Genegun.htm<http://thalamus.wustl.edu/nonetlab/ResourcesF/genegun/Genegun.htm>
>>>>>>>> http://dictybase.org/**techniques/particle%20gun/**particle_gun.htm<http://dictybase.org/techniques/particle%20gun/particle_gun.htm>
>>>>>>>> http://diagnosisp.com/dp/**journals/view_pdf.php?journal_**
>>>>>>>> id=1&archive=0&issue_id=5&**article_id=148<http://diagnosisp.com/dp/journals/view_pdf.php?journal_id=1&archive=0&issue_id=5&article_id=148>
>>>>>>>>
>>>>>>>>
>>>>>>>>
>>>>>>>>
>>>>>>>> On Monday, August 27, 2012 3:03:45 AM UTC-7, Patrik D'haeseleer wrote:
>>>>>>>>>
>>>>>>>>> We were tossing around the idea of a DIY gene gun at BioCurious as
>>>>>>>>> well. Sure, helium and gold particles may be more efficient, but I bet a
>>>>>>>>> hacked bb gun and, say, magnetite particles may give at least some
>>>>>>>>> transformation as well.
>>>>>>>>>
>>>>>>>>> Here's a great video on how to prepare DNA "bullets", and how to use
>>>>>>>>> the gene gun to transform a small selection of cells in live neuronal
>>>>>>>>> samples:
>>>>>>>>>
>>>>>>>>> http://www.jove.com/index/**deta**ils.stp?id=675<http://www.jove.com/index/details.stp?id=675>
>>>>>>>>>
>>>>>>>>> Patrik
>>>>>>>>>
>>>>>>>>> On Sunday, August 26, 2012 3:15:53 AM UTC-7, Mega wrote:
>>>>>>>>>>
>>>>>>>>>> Hi,
>>>>>>>>>>
>>>>>>>>>> I 'm wondering how one could build such a device for particle
>>>>>>>>>> bombardement.
>>>>>>>>>>
>>>>>>>>>> They usually use Helium, but why not Nitrogen or Carbon dioxide
>>>>>>>>>> (widely available). I read they use low pressure, but what is low?
>>>>>>>>>> Does it have to be in a vakuum??? I can do this, but it will be
>>>>>>>>>> expensive and difficult to build the vakuum-tight casing and buy the vakuum
>>>>>>>>>> pump.
>>>>>>>>>>
>>>>>>>>>> Can there be too much pressure (so the particles fly through the
>>>>>>>>>> cells without leaving DNA)? Or can I configure it just using 'some
>>>>>>>>>> pressure' ?
>>>>>>>>>>
>>>>>>>>>>
>>>>>>>>>> (I have to look up the laws of my country, but as you cannot harm
>>>>>>>>>> anyone with this device, it shouldn't be an issue)
>>>>>>>>>>
>>>>>>>>>>
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>>>>>
>>>>
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>>>
>>
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>
>
>
> --
> Nathan McCorkle
> Rochester Institute of Technology
> College of Science, Biotechnology/Bioinformatics



--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics

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