Re: [DIYbio] Re: Building a particle bombardement device DIY

Its a magnetic field, not electric. The magnetic particles with DNA
bound are suspended evenly in the cell, and you apply a magnetic pulse
in one direction (I guess a changing field could work too). Think
magetic bead DNA separation kits, where you just place a magnet on the
side of a microfuge tube, now add some unexpecting cells to get in the
way, and quickly bring the strong magnet to the side of the tube...
some magnetic beads will rip through a cell all the way to make it to
the tube edge, some magnetic particles will get stuck mid-way through
the cell.

I heard a guy give a talk on microfluidics who used to work at Kodak,
and now is at SUNY Buffalo, he has done some of this magnetofection
stuff
http://www.cbe.buffalo.edu/people/full_time/e_furlani.php

On Tue, Aug 28, 2012 at 5:23 PM, Cathal Garvey <cathalgarvey@gmail.com> wrote:
> I suppose if one were to apply a rapid AC field to the cells, it could
> "shake" the nanoparticles through the membrane, but that field would
> probably have to be strong enough that it's hard to distinguish it from
> regular chemically assisted electroporation.
>
> Still, I've never tried it. Maybe the efficiencies are indeed crazy high
> and toxicities low, as it reads in the article.
>
> On 28/08/12 19:39, Nathan McCorkle wrote:
>> On Tue, Aug 28, 2012 at 2:33 PM, Cathal Garvey <cathalgarvey@gmail.com> wrote:
>>> Magnetofection's article reads like an advert.
>>
>> hmm, i don't see it that way
>>
>>>
>>> Also, if the article is to be believed, it sounds like it's more or less
>>> 100% based on chemical transformation, with the "magneto" part only
>>
>> I also don't see it that way... " Their association with the gene
>> vectors (DNA, siRNA, ODN, virus, etc.) is achieved by salt-induced
>> colloidal aggregation and electrostatic interaction" then you apply an
>> electromagnetic field and the magnet:DNA complex rips into the cell.
>>
>> More like electroporation or gene gun to me than chemical methods
>>
>>> describing the means of concentrating the transforming material close to
>>> the cells. The particles are otherwise just DNA coated in "proprietary
>>> formulations", probably some blend of PEG and molecules with some
>>> affinity for cell surface antigens.
>>>
>>> On 28/08/12 18:52, Nathan McCorkle wrote:
>>>> you could also try magnetofection or magnetoporation... some term like
>>>> that...
>>>>
>>>> http://en.wikipedia.org/wiki/Magnetofection
>>>>
>>>>
>>>> On Aug 27, 2012 2:35 PM, "Patrik D'haeseleer" <patrikd@gmail.com> wrote:
>>>>
>>>>> That's a great find!
>>>>>
>>>>> I think we'll just have to experiment a bit to find the right particle
>>>>> composition. Obviously, you want something that is relatively inert,
>>>>> preferably high density, and very small particle size. So for a
>>>>> professional lab, gold or tungsten nanoparticles are an obvious choice.
>>>>> That does *not* mean this is the only way to get it to work though!
>>>>>
>>>>> I brought up magnetite particles before, because I'd been looking into how
>>>>> to make ferrofluid lately. It turns out there's a simple chemical route to
>>>>> make magnetite nanoparticles that are way smaller than the magnetite powder
>>>>> I had bought online in initially. And magnetite may not be as heavy as
>>>>> gold, but I can tell you that jar of magnetite has some serious heft to it.
>>>>> Probably worth a try. Plus, the magnetic nature of the particles may allow
>>>>> us to manipulate them in ways you can't do with gold.
>>>>>
>>>>> Patrik
>>>>>
>>>>> On Monday, August 27, 2012 8:38:46 AM UTC-7, Mega wrote:
>>>>>>
>>>>>> I was thinking in investing into http://www.alibaba.com/**
>>>>>> product-gs/226547546/Portable_**Gene_Gun.html<http://www.alibaba.com/product-gs/226547546/Portable_Gene_Gun.html>this one. 300$ for one device.
>>>>>>
>>>>>>
>>>>>> But if the particles are this expensive...
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>> On Mon, Aug 27, 2012 at 12:22 PM, Patrik D'haeseleer <pat...@gmail.com>wrote:
>>>>>>
>>>>>>> Here's some more useful DIY info:
>>>>>>>
>>>>>>> http://thalamus.wustl.edu/**nonetlab/ResourcesF/genegun/**Genegun.htm<http://thalamus.wustl.edu/nonetlab/ResourcesF/genegun/Genegun.htm>
>>>>>>> http://dictybase.org/**techniques/particle%20gun/**particle_gun.htm<http://dictybase.org/techniques/particle%20gun/particle_gun.htm>
>>>>>>> http://diagnosisp.com/dp/**journals/view_pdf.php?journal_**
>>>>>>> id=1&archive=0&issue_id=5&**article_id=148<http://diagnosisp.com/dp/journals/view_pdf.php?journal_id=1&archive=0&issue_id=5&article_id=148>
>>>>>>>
>>>>>>>
>>>>>>>
>>>>>>>
>>>>>>> On Monday, August 27, 2012 3:03:45 AM UTC-7, Patrik D'haeseleer wrote:
>>>>>>>>
>>>>>>>> We were tossing around the idea of a DIY gene gun at BioCurious as
>>>>>>>> well. Sure, helium and gold particles may be more efficient, but I bet a
>>>>>>>> hacked bb gun and, say, magnetite particles may give at least some
>>>>>>>> transformation as well.
>>>>>>>>
>>>>>>>> Here's a great video on how to prepare DNA "bullets", and how to use
>>>>>>>> the gene gun to transform a small selection of cells in live neuronal
>>>>>>>> samples:
>>>>>>>>
>>>>>>>> http://www.jove.com/index/**deta**ils.stp?id=675<http://www.jove.com/index/details.stp?id=675>
>>>>>>>>
>>>>>>>> Patrik
>>>>>>>>
>>>>>>>> On Sunday, August 26, 2012 3:15:53 AM UTC-7, Mega wrote:
>>>>>>>>>
>>>>>>>>> Hi,
>>>>>>>>>
>>>>>>>>> I 'm wondering how one could build such a device for particle
>>>>>>>>> bombardement.
>>>>>>>>>
>>>>>>>>> They usually use Helium, but why not Nitrogen or Carbon dioxide
>>>>>>>>> (widely available). I read they use low pressure, but what is low?
>>>>>>>>> Does it have to be in a vakuum??? I can do this, but it will be
>>>>>>>>> expensive and difficult to build the vakuum-tight casing and buy the vakuum
>>>>>>>>> pump.
>>>>>>>>>
>>>>>>>>> Can there be too much pressure (so the particles fly through the
>>>>>>>>> cells without leaving DNA)? Or can I configure it just using 'some
>>>>>>>>> pressure' ?
>>>>>>>>>
>>>>>>>>>
>>>>>>>>> (I have to look up the laws of my country, but as you cannot harm
>>>>>>>>> anyone with this device, it shouldn't be an issue)
>>>>>>>>>
>>>>>>>>>
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>>>>
>>>
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--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics

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