Re: [DIYbio] Re: Building a particle bombardement device DIY

I suppose if one were to apply a rapid AC field to the cells, it could
"shake" the nanoparticles through the membrane, but that field would
probably have to be strong enough that it's hard to distinguish it from
regular chemically assisted electroporation.

Still, I've never tried it. Maybe the efficiencies are indeed crazy high
and toxicities low, as it reads in the article.

On 28/08/12 19:39, Nathan McCorkle wrote:
> On Tue, Aug 28, 2012 at 2:33 PM, Cathal Garvey <cathalgarvey@gmail.com> wrote:
>> Magnetofection's article reads like an advert.
>
> hmm, i don't see it that way
>
>>
>> Also, if the article is to be believed, it sounds like it's more or less
>> 100% based on chemical transformation, with the "magneto" part only
>
> I also don't see it that way... " Their association with the gene
> vectors (DNA, siRNA, ODN, virus, etc.) is achieved by salt-induced
> colloidal aggregation and electrostatic interaction" then you apply an
> electromagnetic field and the magnet:DNA complex rips into the cell.
>
> More like electroporation or gene gun to me than chemical methods
>
>> describing the means of concentrating the transforming material close to
>> the cells. The particles are otherwise just DNA coated in "proprietary
>> formulations", probably some blend of PEG and molecules with some
>> affinity for cell surface antigens.
>>
>> On 28/08/12 18:52, Nathan McCorkle wrote:
>>> you could also try magnetofection or magnetoporation... some term like
>>> that...
>>>
>>> http://en.wikipedia.org/wiki/Magnetofection
>>>
>>>
>>> On Aug 27, 2012 2:35 PM, "Patrik D'haeseleer" <patrikd@gmail.com> wrote:
>>>
>>>> That's a great find!
>>>>
>>>> I think we'll just have to experiment a bit to find the right particle
>>>> composition. Obviously, you want something that is relatively inert,
>>>> preferably high density, and very small particle size. So for a
>>>> professional lab, gold or tungsten nanoparticles are an obvious choice.
>>>> That does *not* mean this is the only way to get it to work though!
>>>>
>>>> I brought up magnetite particles before, because I'd been looking into how
>>>> to make ferrofluid lately. It turns out there's a simple chemical route to
>>>> make magnetite nanoparticles that are way smaller than the magnetite powder
>>>> I had bought online in initially. And magnetite may not be as heavy as
>>>> gold, but I can tell you that jar of magnetite has some serious heft to it.
>>>> Probably worth a try. Plus, the magnetic nature of the particles may allow
>>>> us to manipulate them in ways you can't do with gold.
>>>>
>>>> Patrik
>>>>
>>>> On Monday, August 27, 2012 8:38:46 AM UTC-7, Mega wrote:
>>>>>
>>>>> I was thinking in investing into http://www.alibaba.com/**
>>>>> product-gs/226547546/Portable_**Gene_Gun.html<http://www.alibaba.com/product-gs/226547546/Portable_Gene_Gun.html>this one. 300$ for one device.
>>>>>
>>>>>
>>>>> But if the particles are this expensive...
>>>>>
>>>>>
>>>>>
>>>>>
>>>>>
>>>>> On Mon, Aug 27, 2012 at 12:22 PM, Patrik D'haeseleer <pat...@gmail.com>wrote:
>>>>>
>>>>>> Here's some more useful DIY info:
>>>>>>
>>>>>> http://thalamus.wustl.edu/**nonetlab/ResourcesF/genegun/**Genegun.htm<http://thalamus.wustl.edu/nonetlab/ResourcesF/genegun/Genegun.htm>
>>>>>> http://dictybase.org/**techniques/particle%20gun/**particle_gun.htm<http://dictybase.org/techniques/particle%20gun/particle_gun.htm>
>>>>>> http://diagnosisp.com/dp/**journals/view_pdf.php?journal_**
>>>>>> id=1&archive=0&issue_id=5&**article_id=148<http://diagnosisp.com/dp/journals/view_pdf.php?journal_id=1&archive=0&issue_id=5&article_id=148>
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>> On Monday, August 27, 2012 3:03:45 AM UTC-7, Patrik D'haeseleer wrote:
>>>>>>>
>>>>>>> We were tossing around the idea of a DIY gene gun at BioCurious as
>>>>>>> well. Sure, helium and gold particles may be more efficient, but I bet a
>>>>>>> hacked bb gun and, say, magnetite particles may give at least some
>>>>>>> transformation as well.
>>>>>>>
>>>>>>> Here's a great video on how to prepare DNA "bullets", and how to use
>>>>>>> the gene gun to transform a small selection of cells in live neuronal
>>>>>>> samples:
>>>>>>>
>>>>>>> http://www.jove.com/index/**deta**ils.stp?id=675<http://www.jove.com/index/details.stp?id=675>
>>>>>>>
>>>>>>> Patrik
>>>>>>>
>>>>>>> On Sunday, August 26, 2012 3:15:53 AM UTC-7, Mega wrote:
>>>>>>>>
>>>>>>>> Hi,
>>>>>>>>
>>>>>>>> I 'm wondering how one could build such a device for particle
>>>>>>>> bombardement.
>>>>>>>>
>>>>>>>> They usually use Helium, but why not Nitrogen or Carbon dioxide
>>>>>>>> (widely available). I read they use low pressure, but what is low?
>>>>>>>> Does it have to be in a vakuum??? I can do this, but it will be
>>>>>>>> expensive and difficult to build the vakuum-tight casing and buy the vakuum
>>>>>>>> pump.
>>>>>>>>
>>>>>>>> Can there be too much pressure (so the particles fly through the
>>>>>>>> cells without leaving DNA)? Or can I configure it just using 'some
>>>>>>>> pressure' ?
>>>>>>>>
>>>>>>>>
>>>>>>>> (I have to look up the laws of my country, but as you cannot harm
>>>>>>>> anyone with this device, it shouldn't be an issue)
>>>>>>>>
>>>>>>>>
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>>
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