Re: [DIYbio] Re: DIY media for bioluminescente algae

Note that the Micro Algae Grow Mass Pack link I posted earlier *is* f/2 medium. At $20 for 10,000 liter, there's really no reason to mix your own or use plant fertilizer...

Dinoflagellates grow very slowly, doubling every 5-6 days at most, so they typically don't need much CO2. Plus they don't like constant motion. For a large volume like 100 liter, maybe get an aquarium pump that you turn on once a day or so. Heck, with some luck you might be able to find a cheap second hand 100L aquarium on craigslist, complete with light fixtures and pump. (You'll definitely also need some grow lights on a timer, if you want the dinos to "perform" on schedule.)

Patrik

On Tuesday, August 28, 2012 3:19:53 AM UTC-7, Loïc wrote:

Hi,

Thanks for answers,

I did a success trial with seawater 3 months ago, but i had a problem of contaminant (green algae) and the concentration didn't satisfied me.
I will  create a bioreactor with at least 100 liters and more later for a visual performance, so i need a large volume in a transparent box and the higher concentration i can.
I will try with plant fertilizer, (thanks Patrick), and try to reproduce the exact f2 medium composition for accurate reproduction.
is there a problem to provide co2 in a large volume ?

see you

Loïc

2012/8/28 Patrik D'haeseleer <pat...@gmail.com>

Plant fertilizers are great if the goal is to be able to tell any ten year old how to grow their own dinos, but if you're growing them yourself in massive amounts (love to hear how massive, and why!), you're much better off buying the Micro Algae Grow Mass Pack (without silicate) from here:

http://florida-aqua-farms.com/secure/agora.cgi?product=MICRO_MACRO_NUTRIENTS

$20 plus shipping will buy you a whole kilo of dry nutrients (just add salt water), which is enough for 10,000 liters! At that rate, you'll probably pay a lot more to filter or sterilize the salt water than on the nutrient mix...

Patrik

On Monday, August 27, 2012 2:59:48 PM UTC-7, Patrik D'haeseleer wrote:

The official medium (L1 or f/2) is mostly nitrate by weight, so we figured we'd give plant fertilizers a try. I like liquid Miracle-Gro, because it's easiest to find and work with, but pretty much everything we've tried works. You just get more trouble with cyanobacterial contamination at higher fertilizer concentrations, presumably because the phosphorus content is much higher than in the official medium.

We used salt water from a local aquarium store, but you should be able to avoid contamination issues by sterilizing the water, or mixing your own from filtered water plus sea salts.

On Monday, August 27, 2012 2:55:05 PM UTC-7, Patrik D'haeseleer wrote:

We've been doing lots of DIY growth experiments with P. lunula at BioCurious lately. In fact, we handed out around 250 vials with dinoflagellates for people to take home at Maker Faire a coupel months ago, along with this "Care & Feeding" link:

http:// TinyUrl.com / MyPetDinos

(To be updated with more recent results soon.)

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