On Tuesday, August 28, 2012 10:15:36 AM UTC-7, Cathal wrote:
Would magnetite not aggregate into lumps?
Yup - that's why the ferrofluid recipe uses oleic acid as a surfactant to keep the magnetite nanoparticles separated and suspended in the carrier oil. There's actually some water soluble ferrofluids as well, for use in some biomedical applications:
http://ferrofluid.ferrotec.com/products/ferrofluid/emg/water/
--
On 27/08/12 19:35, Patrik D'haeseleer wrote:
> That's a great find!
>
> I think we'll just have to experiment a bit to find the right particle
> composition. Obviously, you want something that is relatively inert,
> preferably high density, and very small particle size. So for a
> professional lab, gold or tungsten nanoparticles are an obvious choice.
> That does *not* mean this is the only way to get it to work though!
>
> I brought up magnetite particles before, because I'd been looking into how
> to make ferrofluid lately. It turns out there's a simple chemical route to
> make magnetite nanoparticles that are way smaller than the magnetite powder
> I had bought online in initially. And magnetite may not be as heavy as
> gold, but I can tell you that jar of magnetite has some serious heft to it.
> Probably worth a try. Plus, the magnetic nature of the particles may allow
> us to manipulate them in ways you can't do with gold.
>
> Patrik
>
> On Monday, August 27, 2012 8:38:46 AM UTC-7, Mega wrote:
>>
>> I was thinking in investing into
>> http://www.alibaba.com/product-gs/226547546/Portable_ thisGene_Gun.html
>> one. 300$ for one device.
>>
>>
>> But if the particles are this expensive...
>>
>>
>>
>>
>>
>> On Mon, Aug 27, 2012 at 12:22 PM, Patrik D'haeseleer <pat...@gmail.com<javascript:>
>>> wrote:
>>
>>> Here's some more useful DIY info:
>>>
>>> http://thalamus.wustl.edu/nonetlab/ResourcesF/genegun/ Genegun.htm
>>> http://dictybase.org/techniques/particle%20gun/ particle_gun.htm
>>>
>>> http://diagnosisp.com/dp/journals/view_pdf.php?journal_ id=1&archive=0&issue_id=5& article_id=148
>>>
>>>
>>>
>>>
>>> On Monday, August 27, 2012 3:03:45 AM UTC-7, Patrik D'haeseleer wrote:
>>>>
>>>> We were tossing around the idea of a DIY gene gun at BioCurious as well.
>>>> Sure, helium and gold particles may be more efficient, but I bet a hacked
>>>> bb gun and, say, magnetite particles may give at least some transformation
>>>> as well.
>>>>
>>>> Here's a great video on how to prepare DNA "bullets", and how to use the
>>>> gene gun to transform a small selection of cells in live neuronal samples:
>>>>
>>>> http://www.jove.com/index/**details.stp?id=675 <http://www.jove.com/index/details.stp?id= >675
>>>>
>>>> Patrik
>>>>
>>>> On Sunday, August 26, 2012 3:15:53 AM UTC-7, Mega wrote:
>>>>>
>>>>> Hi,
>>>>>
>>>>> I 'm wondering how one could build such a device for particle
>>>>> bombardement.
>>>>>
>>>>> They usually use Helium, but why not Nitrogen or Carbon dioxide (widely
>>>>> available). I read they use low pressure, but what is low?
>>>>> Does it have to be in a vakuum??? I can do this, but it will be
>>>>> expensive and difficult to build the vakuum-tight casing and buy the vakuum
>>>>> pump.
>>>>>
>>>>> Can there be too much pressure (so the particles fly through the cells
>>>>> without leaving DNA)? Or can I configure it just using 'some pressure' ?
>>>>>
>>>>>
>>>>> (I have to look up the laws of my country, but as you cannot harm
>>>>> anyone with this device, it shouldn't be an issue)
>>>>>
>>>>>
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>>
>>
>
--
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