[DIYbio] Re: Looking for Chloroplast integration Vector

I am just guessing that your goal is high expression of lux. If this is the case, I am afraid you are not going to get any higher expression by doubling the genes. There are hundreds of copies of chloroplast genome per each chloroplast and hundreds or even thousands chloroplasts per cell. So in most cases your expression level is not limited by low gene load or inefficient transcription, but by the protein stability and related issues.
The double casette also considerable increases the probability of homologous recombination. Basically the same process you are trying to take advantage of to integrate your DNA into chloroplast genome is going to cut out better part of your gene of interest.
 


--
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/0H5Pb0STzVIJ.
For more options, visit https://groups.google.com/groups/opt_out.
 
 

  • Digg
  • Del.icio.us
  • StumbleUpon
  • Reddit
  • RSS

0 comments:

Post a Comment