Re: [DIYbio] 3D printing medical devices

On Mon, Oct 1, 2012 at 7:31 PM, Jose Gomez-Marquez
<jose.gomez.marquez@gmail.com> wrote:
> Great stuff on the bioprinting Patrik. We have been wondering about the
> inkshield as an alternative to automatic pipettors.
>
> In reference to the medical devices as DIYBio. I am going to put a flag up
> and say YES.
[snip a collection of plausible relations but with no citation of
anybody's formal definition in a peer-reviewed source.]

This came up as a question for what should be included in the
Wikipedia articles. I'd like to be able to cite your e-mail to this
list as a reliable source, but Wikipedia guidelines and policies about
reliable sources just don't extend that far. Wikipedia isn't a place
to define terms. It's a place to describe what others have written
about, in sources that meet certain criteria.

Regards,
Michael Turner
Project Persephone
1-25-33 Takadanobaba
Shinjuku-ku Tokyo 169-0075
(+81) 90-5203-8682
turner@projectpersephone.org
http://www.projectpersephone.org/

"Love does not consist in gazing at each other, but in looking outward
together in the same direction." -- Antoine de Saint-Exupéry


>
> On Monday, October 1, 2012 3:09:01 AM UTC-4, Patrik D'haeseleer wrote:
>>
>> The bottom CD head plus the platform moves along one axis, the top CD head
>> plus the inkjet cartridge moves along the second axis.
>>
>> We haven't yet dealt with how to add a Z axis to this setup, but
>> considering that the inkjet can spray over quite a distance, we should be
>> able to just print several layers over each other without moving Z. There's
>> plenty of interesting stuff to be done in thin layers...
>>
>> The stepper motors driving the CD heads are only 20 steps/revolution. I've
>> heard mythical stories of 200 steps/rev steppers in CD drives, but I've
>> never seen them - maybe in DVD or BlueRay drives? The worm gear on the
>> stepper has a pitch of 3 mm, so each full step moves the head by 150 micron.
>> We should theoretically be able to improve that to <10 micron with 16x
>> microstepping, but we haven't had a need for that yet (we'd also have to
>> switch out the drivers to do microstepping). The 12 nozzles on the inkjet
>> cartridge are 96 dpi, or 265 micrometers apart. Printing with ink, the
>> individual dots are clearly distinguishable, so they should be well under
>> 265 micron, but we haven't measured them yet - that's our real resolution
>> limit at the moment, not so much the XY positioning.
>>
>> Printing at 10 micron or somesuch sounds very appealing, but it's really a
>> huge overkill for most applications. The number of cells per droplet will be
>> stochastic anyway, so it's not as if you can put cells *exactly* where you
>> want them. And dividing the diameter of the droplets by 10 just means that
>> it'll take you 1000 times longer to print a given volume...
>>
>>
>> On Sunday, September 30, 2012 6:22:05 PM UTC-7, wgh...@gmail.com wrote:
>>>
>>> Wow man!!! My kingdom for a video. Is this cartridge moving or the
>>> platform moving or both? You guys rock!!!! My other quick question is at
>>> some point your going to have to make a well with a submerged platform that
>>> moves in the z direction. Have you given any thought as to how you will
>>> accomplish this? What is the "dpi" of this current setup?
>>>
>>> -Tim
>>>
>>> P.S.
>>>
>>> I wish there was some easier way to work with the print cartridge. Why
>>> is working with a print cartridge so very complex?!!!
>>>
>>> On Sun, Sep 30, 2012 at 10:37 AM, Patrik D'haeseleer <pat...@gmail.com>
>>> wrote:
>>>>
>>>> Here's a good view of our printer, after we had first gotten it to print
>>>> (in ink):
>>>>
>>>>
>>>> https://secure.flickr.com/photos/68942898@N05/7175637623/in/pool-bioprinter
>>>>
>>>> The grey rectangle on top is one of the linear actuators we scavenged
>>>> out of the CD drives. It's got a little stepper motor with a worm gear,
>>>> moving the CD read head back and forth. The inkjet cartridge is mounted on
>>>> the read head. We had to cut out some of the grey frame to allow the
>>>> cartridge to move the full distance (only 2 inch or so - the radius of a
>>>> CD).
>>>>
>>>> There's another one of these linear actuators on the bottom, at a 90
>>>> degree angle. The printing platform (in this case, a post-it note) sits on
>>>> top of the read head there. Only the read heads and what's attached to them
>>>> are moving: the bottom head is moving front to back with the post-it, the
>>>> top head is moving left to right with the print head.
>>>>
>>>> We'll have some better pics for you guys later - hoping to put together
>>>> an Instructable on this soon.
>>>>
>>>> Patrik
>>>>
>>>>
>>>> On Sunday, September 30, 2012 1:08:29 AM UTC-7, wgh...@gmail.com wrote:
>>>>>
>>>>> Hi Again,
>>>>>
>>>>> Its like drugs to me to hear about this project. I appreciate your
>>>>> continued assistance in helping me understand more about your project. In
>>>>> the XY configuration, where was the inkjet cartridge, was it attached in
>>>>> such a way that was independent of the printer? I can't explain why I want
>>>>> to do this, but I just do :> I will keep asking questions until you won't
>>>>> let me anymore or they kick me off the list :>
>>>>>
>>>>> -Tim
>>>>>
>>>>> On Fri, Sep 28, 2012 at 12:28 AM, Patrik D'haeseleer <pat...@gmail.com>
>>>>> wrote:
>>>>>>
>>>>>> Heh - the food coloring (and the arabinose) was just with an old
>>>>>> inkjet printer we rescued from the sidewalk. Cut off the top of the
>>>>>> cartridge, rinse out thoroughly, fill with water + food coloring, print test
>>>>>> page.
>>>>>>
>>>>>> For our second generation bioprinter, we built an XY-platform from the
>>>>>> read head mechanism from two old CD drives (need to search around for the
>>>>>> type that uses a stepper motor), driven by an Arduino. This part was
>>>>>> entirely inspired by Hackteria's work along these lines:
>>>>>>
>>>>>>
>>>>>> http://hackteria.org/wiki/index.php/HackteriaLab_2011_Commons#Micro_Manipulator
>>>>>>
>>>>>>
>>>>>> http://hackteria.org/wiki/index.php/DIY_Micro_Dispensing_and_Bio_Printing
>>>>>>
>>>>>> http://hackteria.org/wiki/index.php/DIY_Micro_Laser_Cutter
>>>>>>
>>>>>>
>>>>>> http://hackteria.org/wiki/index.php/DIY_Microfluidics#Advanced_DIY_Microfluidics
>>>>>>
>>>>>> http://hackteria.org/?p=1186
>>>>>>
>>>>>> And I think they got their idea from these guys:
>>>>>>
>>>>>>
>>>>>> http://builders.reprap.org/2010/08/selective-laser-sintering-part-8.html
>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>> On Friday, September 28, 2012 2:12:52 AM UTC-7, wgh...@gmail.com
>>>>>> wrote:
>>>>>>>
>>>>>>> Wow, this is the most real info on bio printing I have ever come
>>>>>>> across thanks! I have a couple more quick questions for you. When you used
>>>>>>> the food coloring did you just print it onto paper? What are you using for
>>>>>>> x-y positioning of the printer head? Are you printing to a well or what are
>>>>>>> you actually printing on? Somehow you ended up with something in a petri
>>>>>>> dish but I am confused how you got it in there. Can you please elaborate?
>>>>>>> I am quite excited by your progress.
>>>>>>>
>>>>>>> -Tim
>>>>>>>
>>>>>>> P.S.
>>>>>>>
>>>>>>> The main issue as I understand it is positioning. It sounds like
>>>>>>> your using cd motors of some kind to solve this issue. Can you elaborate on
>>>>>>> how your solving this issue? What is the printing resolution needed for
>>>>>>> diybio cell printing in your opinion? Is 96 dpi too much or not enough
>>>>>>> etc...? Makerbot appears to have solved much of the issues your probably
>>>>>>> facing, have you looked at adapting that platform to bio printing just
>>>>>>> curious?
>>>>>>>
>>>>>>> On Thu, Sep 27, 2012 at 9:43 PM, Patrik D'haeseleer
>>>>>>> <pat...@gmail.com> wrote:
>>>>>>>>
>>>>>>>>
>>>>>>>>
>>>>>>>> On Thursday, September 27, 2012 3:40:56 PM UTC-7, wgh...@gmail.com
>>>>>>>> wrote:
>>>>>>>>>
>>>>>>>>>
>>>>>>>>> I have been very interested in doing bio printing. I tried to get
>>>>>>>>> an old hp deskjet printer and make it print a jello like substance but
>>>>>>>>> couldn't even do that.
>>>>>>>>
>>>>>>>>
>>>>>>>> Have you tried just printing with food coloring in water? That is
>>>>>>>> the first thing we did, and that worked fine. One problem you may be having
>>>>>>>> with a gel is that the inkjet head expects a fluid of a certain viscosity.
>>>>>>>> Plus as the thermal inkjet vaporizes some of the ink, some gelatin may burn
>>>>>>>> and eventually clog the print head.
>>>>>>>>
>>>>>>>> The second thing we tried, after food coloring, was to print with
>>>>>>>> arabinose in water onto filter paper. Then we put the filter paper onto a
>>>>>>>> lawn of E. coli with the pGLO plasmid containing GFP under an arabinose
>>>>>>>> inducible promoter, so wherever we had printed arabinose, we saw the GFP
>>>>>>>> light up:
>>>>>>>>
>>>>>>>>
>>>>>>>> https://secure.flickr.com/photos/68942898@N05/6799091190/in/pool-bioprinter
>>>>>>>>
>>>>>>>> We clearly got a lot of diffusion of the arabinose, but you can
>>>>>>>> still make out the BioCurious logo lighting up in GFP.
>>>>>>>>
>>>>>>>> Cells suspended in liquid should be feasible using an inkjet, but if
>>>>>>>> you really want to print with cells embedded in a gel, you'll probably want
>>>>>>>> to move to a syringe pump system. We're currently looking at mounting a DIY
>>>>>>>> syringe pump (probably driven by another linear actuator from a CD drive) on
>>>>>>>> our BioPrinter.
>>>>>>>>
>>>>>>>>>
>>>>>>>>> I am completely frustrated with how to do this. Can anyone give
>>>>>>>>> me some real help to figure out how to do this for a diybio person? I am
>>>>>>>>> trying to think of what would be a good approach to developing the diybio
>>>>>>>>> bio printer. My idea was that I would start with trying to print a jello
>>>>>>>>> like gel with different colors in the wells to "test" that it is working.
>>>>>>>>> After I complete that task I would then move on to perhaps printing some
>>>>>>>>> plant cells or something. I would then like to print cells that are not
>>>>>>>>> dangerous to work with like human cells. I am curious are bone cells
>>>>>>>>> considered safe? What cells are appropriate for bio printing that would be
>>>>>>>>> safe? I keep looking at the makerbot and thinking in my mind, can't this be
>>>>>>>>> adapted to bio printing? The only thing I see missing is that the platform
>>>>>>>>> needs to move up and down that is being printed on. Isn't that all that is
>>>>>>>>> missing? I guess the resolution may not be high enough either. Anyway, I
>>>>>>>>> would love to get a simple working bio printer up and working, help me ob1,
>>>>>>>>> YOUR MY ONLY HOPE! :>
>>>>>>>>>
>>>>>>>>> -Tim
>>>>>>>>>
>>>>>>>>> P.S.
>>>>>>>>>
>>>>>>>>> I turn cell phones into robots. I am very familiar with arduinos,
>>>>>>>>> laser printing, serial communication. You can see my latest robot here:
>>>>>>>>>
>>>>>>>>> http://www.youtube.com/watch?v=E_q5WD3dTkE
>>>>>>>>>
>>>>>>>>> On Wed, Sep 26, 2012 at 12:12 PM, kingjacob <king...@gmail.com>
>>>>>>>>> wrote:
>>>>>>>>>>
>>>>>>>>>> You can print in titanium using Direct Metal Laser Sintering. You
>>>>>>>>>> can pretty much print with any material you can turn into a powder or resin.
>>>>>>>>>> A friend of mine (papers below) even used a NaCl mixture to 3D print tissue
>>>>>>>>>> scaffolding.
>>>>>>>>>>
>>>>>>>>>> Computer-aided tissue engineering: benefiting from the control
>>>>>>>>>> over scaffold micro-architecture.
>>>>>>>>>>
>>>>>>>>>> http://www.ncbi.nlm.nih.gov/pubmed/22692601
>>>>>>>>>>
>>>>>>>>>> Scaffold pore space modulation through intelligent design of
>>>>>>>>>> dissolvable microparticles.
>>>>>>>>>>
>>>>>>>>>> http://www.ncbi.nlm.nih.gov/pubmed/22692605
>>>>>>>>>>
>>>>>>>>>> Computer-aided tissue engineering of a human vertebral body.
>>>>>>>>>>
>>>>>>>>>> http://www.ncbi.nlm.nih.gov/pubmed/16240082
>>>>>>>>>>
>>>>>>>>>> On Wed, Sep 26, 2012 at 4:45 PM, Nathan McCorkle
>>>>>>>>>> <nmz...@gmail.com> wrote:
>>>>>>>>>>>
>>>>>>>>>>> I've never heard anyone on here discussing printable hip
>>>>>>>>>>> replacements... it sounds like a bad idea anyway, seeing as how
>>>>>>>>>>> poor
>>>>>>>>>>> 3D printer plastics fair in strength. Most joint replacements are
>>>>>>>>>>> made
>>>>>>>>>>> of titanium, etc... Who put that in there?
>>>>>>>>>>>
>>>>>>>>>>> It also has really old info scattered throughout, and doesn't
>>>>>>>>>>> mention
>>>>>>>>>>> anything of the years of FBI interaction we've had
>>>>>>>>>>>
>>>>>>>>>>> On Tue, Sep 25, 2012 at 9:02 PM, Bryan Bishop <kan...@gmail.com>
>>>>>>>>>>> wrote:
>>>>>>>>>>> > The wikipedia articles are still awful.
>>>>>>>>>>> >
>>>>>>>>>>> > http://en.wikipedia.org/wiki/Biohacking
>>>>>>>>>>> > http://en.wikipedia.org/wiki/DIYbio
>>>>>>>>>>> > http://en.wikipedia.org/wiki/Biopunk
>>>>>>>>>>> >
>>>>>>>>>>> > At least that last one is somewhat less awful. Anyone want to
>>>>>>>>>>> > take cleanup
>>>>>>>>>>> > duty?
>>>>>>>>>>> >
>>>>>>>>>>> > - Bryan
>>>>>>>>>>> > http://heybryan.org/
>>>>>>>>>>> > 1 512 203 0507
>>>>>>>>>>> >
>>>>>>>>>>> > --
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>>>>>>>>>>>
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>>>>>>>>>>>
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>>>>>>>>>>> >
>>>>>>>>>>> >
>>>>>>>>>>>
>>>>>>>>>>>
>>>>>>>>>>>
>>>>>>>>>>> --
>>>>>>>>>>> Nathan McCorkle
>>>>>>>>>>> Rochester Institute of Technology
>>>>>>>>>>> College of Science, Biotechnology/Bioinformatics
>>>>>>>>>>>
>>>>>>>>>>> --
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>>>>>>>>>>>
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>>>>>>>>>>>
>>>>>>>>>>>
>>>>>>>>>>
>>>>>>>>>>
>>>>>>>>>>
>>>>>>>>>> --
>>>>>>>>>> Cheers,
>>>>>>>>>> Jacob Shiach
>>>>>>>>>> editor-in-chief: Citizen Science Quarterly
>>>>>>>>>> founder: Brightwork CoResearch
>>>>>>>>>> twitter: @jacobshiach
>>>>>>>>>>
>>>>>>>>>> --
>>>>>>>>>> You received this message because you are subscribed to the Google
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>>>>>>>>>> To post to this group, send email to diy...@googlegroups.com.
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>>>>>>>>>> diybio+un...@googlegroups.com.
>>>>>>>>>>
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>>>>>>>>>>
>>>>>>>>>>
>>>>>>>>>
>>>>>>>>>
>>>>>>>> --
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>>>>>>>>
>>>>>>>>
>>>>>>>
>>>>>>>
>>>>>> --
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>>>>>>
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>>>>>>
>>>>>>
>>>>>
>>>>>
>>>> --
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>>>>
>>>>
>>>
>>>
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