Re: [DIYbio] Re: DNA design questions

The longer the chain, the more chances there are for a weak link to
break it.

Issues of transcriptional stalling or translational abortion/stalling
could end up killing the whole system, and it's hard to debug. At least
if you have several smaller fusions, you can try to identify which part
of the system is failing and fix just that.

I'd suggest several smaller fusion proteins rather than one giant one.
Fuse the critical pairs or sets that form "bottlenecks" in the system:
LuxAB are cofactors, so fuse them. LuxCDE are steps in a synthetic
chain, so fuse them.

On 28/10/12 16:50, Mega wrote:
> Does anyone by chance know how long an amino acid chain can be without
> getting problems with transcription and/or translation?
>
> Say you want to make a 6kbp long fusion protein (you clone the genes in
> frame and remove the stop codons) . That would make 2000 amino acids(!),
> and those sequences I've seen so far have around 400 amino acids.
>
>
> Will there be problems with the RNA polymerase or during translation?
> (Google didn't hit any adequate results, unfortunately)
>
>
>
>


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