Re: [DIYbio] Re: Now, about that gold gun...

Helium is used because it can achieve higher speeds, but you don't need
that much power to punch through a bit of peptidoglycan/cellulose and a
lipid bilayer. :)

On 28/10/12 19:21, Conner Berthold wrote:
> I was also looking to see if Helium could be replaced with something more
> "portable" (like CO2 cartridges) and found this article.
>
> "With the self-design CO2 propelled gene gun (200
> psi, distance 3 cm with 400 mesh nylon screen) using
> tungsten particle (600 nm diameter) coated with plasmid
> expressing anti-ampicillin gene, the plasmid with antiampicillin gene was
> high efficiently transferred into E.
> Coli cells."(
> http://www.sciencepub.net/nature/0204-supplement/03-mahongbao-schistosome.pdf)(3rd
> page)
>
>
> This link <http://physics.ucsd.edu/~groisman/Gene%20guns.html> also has an
> interesting approach to prevent harm by preventing the shock wave of gas
> from directly hitting the culture. If you could get both of these to work a
> smaller portable gun should be possible.
>


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