Re: [DIYbio] DIY and open Chymosin (Cheese Hacking) - anyone has some more experience

On Mon, Aug 5, 2013 at 6:27 AM, Cathal Garvey
<cathalgarvey@cathalgarvey.me> wrote:
> Hey Marc,
> Would love to work on this, great idea! :)
>
> First question is; how to set up a good antibiotic-free cloning system
> for yeast.

acetamidase has been used, acetamide is super cheap per kg and if
you're using an integration vector, you can probably get away with
selection less often, so your acetamide should last a while.


--
-Nathan

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