[DIYbio] Re: IndieBB Crowdfunding Campaign: Help me make a great beginner's kit for DIYbio/synbio!

So I started wondering last night, do you have any ideas on how to
cure a strain of such a plasmid? I could imagine someone running out
of wild/plasmidless bacteria, or maybe their lab getting contaminated
with the plasmid and thus causing them to have no more plasmidless
E.coli (plasmidless in the sense of your engineered plasmid).

With antibiotic resistance plasmids, you can usually 'cure' them of
the plasmid by growing them without pressure (no antibiotic added) for
several generations. Is there any way to 'cure' these bugs with your
plasmid? Some 'curative' plasmid that interferes with transcription of
the colicin? Seems like to then get rid of the curative plasmid, it
would have to be controlled externally (i.e. with antibiotics).

Thoughts?

On Wed, Jan 22, 2014 at 12:01 PM, Cathal Garvey
<cathalgarvey@cathalgarvey.me> wrote:
> Hey all,
> As anyone on this list for more than a month can attest, our most common
> FAQ here is "How do I get started?". More often than not, it's more
> specifically "How do I get started in synthetic biology?", and our
> answers can get a bit woolly.
>
> The truth is that our options for beginners all suck. Most suppliers are
> hostile towards independent buyers, so we tell new people to buy the
> "refill kit" from Carolina and pretend to be a school, or we mail
> plasmids of dubious provenance to one another. While that's good for
> community spirit, it says a lot that we celebrate knowing the
> approximate sequence of one of them at last.
>
> Finally, we've talked a lot about the issue of getting, using and
> disposing of antibiotics for this purpose a lot, and we've talked more
> and more recently about removing the need for antibiotics altogether.
>
> That's what I aim to do, and I'd really appreciate your help and support
> making it happen. Here's my IndieGoGo campaign, freshly pressed:
> http://www.indiegogo.com/projects/indiebb-your-first-gmo
>
> The kit is designed for beginners, and for teachers and education
> groups, to introduce people to the methods of E.coli engineering. It's
> also designed to be hackable and to be fairly licensed in an Open Source
> way that preserves the user's freedoms going forward. It's fairly
> priced, and designed by and for DIYbioers. It'll be fluorescent, and it
> won't need antibiotics.
>
> If you're interested, please help me out and support the IndieGoGo
> campaign. It's an all-or-nothing campaign, so if I don't hit the goal,
> nothing is raised. If you know a bio/hacker, educator or student who'd
> be interested, please let them know, too. And if you're on Twitter or
> (ugh) Facebook, a shoutout to let others know would be really, really
> appreciated. :)
>
> For the purposes of fundraising and separating my usual noise from the
> campaign, I've started a new twitter account for this, too: @IndieBBDNA
>
> Gratefully yours, and looking forward to collaborating on this and
> making it real!
> Cathal
> PS: As I know too well, nothing can be guaranteed to work in Synbio at
> this point, so bear that in mind. However, this is the most conservative
> design I've yet embarked on, and I'm confident it will work. So, bear
> that in mind too. :)



--
-Nathan

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