Re: [DIYbio] dna purification (once more)

Hi again, I'm going to start a new thread, with pics.

So intro: gram positive bacteria, need to extract chromosomal dna and
its plasmid dna.

Tried so far:
[1] Modified qiagen miniprep (lysozyme treatment before lysis). The
results: Nanodrop concentration of 100ng/uL. The gel image shows a
smear. See attached image.
[2] Used Life Technologies chargeswitch genomic DNA kit.Got about
20ng/uL consistently for 4 samples. Gel shows one band above 10kb (so
maybe 15-20kb?) and another band just on top in wells.

What I need to do:
[3] Since I have total dna, if I can just get the plasmid dna out, I
can then proceed to sequencing. I  am not sure why the plasmid
extraction failed using the miniprep kit. It also failed to purify the
plasmid band on the gel using gel extraction kit.

I hate to keep spending money buying different kits. I want to find
out what exactly is going on.

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