Re: [DIYbio] Re: Ultra-Cheap DNA Printing/Sequencing

On Tue, Feb 25, 2014 at 2:15 PM, John Griessen <john@industromatic.com> wrote:
> On 02/25/2014 03:27 PM, Nathan McCorkle wrote:
>>
>> Luckily I made friends with a guy who has a FIB and underused microfab
>> capable wet lab... So things are almost in place for me to
>> make so leaping progress in the coming months.
>
> Wow! For the rest of you, FIB means focused ion beam, which lets you blast
> away interconnect metal
> in a silicon chip surface if you have it exposed and in the FIB mill vacuum
> chamber. That lets you
> tweak things at low costs compared to new masks for chip fabbing. It might
> also let you carve
> some increased opening in a glass or silicon channel used for microfluidics,
> but probably
> not much use for one that is under glass, (complete and covered), only for
> ones that are half made.

Yep, which is where layering or multi-step processing comes into play.
You could for example make a microfluidic then take it into the FIB to
add nano features. Unfortunately the materials for micro and nano are
often incompatible without more intermediary steps (such as making
negatives and positive reliefs or your 3D surface to change to a
compatible material, or metallization), but if the nano features are
simply a layer of a sandwich, you just have to have good enough
alignment between your sandwich layers. Alternatively there are more
powerful FIB sources (the ion source for milling) that also happen to
be local to me, which can mill from the micro scale all the way to the
nano, while most FIBs slow down tremendously on larger beam spot size
this one actually increases. It's still slow though, compared to
exposing with photolithography and thinking of a nano intermediary
layer, and bonding layers is something require of any microfluidic, so
adding a layer doesn't really require new skills as far as bonding
steps.

With a layered approach, you can use off the shelf nanopores (aka
aperatures, tem windows) or nanotubes if you can get them to easily
polarize and they're dilute enough to be discrete tubes or clustered
groups of tubes... and get away with microlithography using a
microscope and projector or blu-ray writer optical sled. I'd like to
get this out as a kit, with a spin-coater, it will be open-source.


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--
-Nathan

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