Re: [DIYbio] $50 camera good for microscopy with machine vision code included

The software might be nice, but the Canon has higher resolution, lower noise, higher video speeds, and a larger sensor.

That won't matter if all you need is 50 micron (or even 1 micron) resolution. But I've been getting close to 0.2 micron resolution using a Canon T3i (a fairly old camera) and a homemade microscope (I also use an excellent phase-contrast research microscope and get similar resolution but with much less aberration).

When controlled by my computer, I find the Canon to be quite suited to microphotography. You look at the computer monitor, and click on the shutter icon to take the picture. It's pretty friendly.

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On Sat, Jan 31, 2015 at 5:42 AM, John Griessen <john@industromatic.com> wrote:
I was telling Nathan how flex circuits like are used in LCD screens for desktops of a few years ago
are a commodity process of making layers with 50 micron width and space between and might be
repurposed to fabbing microfluidics inexpensively.  I took a flatbed scanner photo of the flex circuit
that could not get down to its resolution, and emailed that, and
offered to send him a piece to look at under a microscope,
since I did not have any good digital camera for microscope photos.

I was saying how I'd take the lens off of a Canon powershot for that, but it still has a crummy user interface
for microphotography, which triggered him telling me about this:


https://www.kickstarter.com/projects/botthoughts/openmv-cam-embedded-machine-vision

It's got a perfect user interface for microphotography -- python programmed machine vision
programs that can recognize features in a scene.  Just add a little problem-specific
development and you have a cell counter program.  Well, add that not-yet-existing, accurate and inexpensive
plastic molded microgrid to drop on your samples then top with a cover slip, *then*
hit the go button of the counting program...

John

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