Pichia pastoris is a species of methylotrophic yeast... "study up", OK?
On Saturday, January 17, 2015 at 7:31:14 AM UTC+5:30, Sunil Phani wrote:
-- On Saturday, January 17, 2015 at 7:31:14 AM UTC+5:30, Sunil Phani wrote:
can someone please help me setup DIY-BIO home Lab to perform mammalian cell culture to produce Recombinant Factor VIII protein with mammalian cells(CHO, HKE 293, HEL, etc) and kindly provide me recombinant dna protocols for identification, isolation , extraction, insertion and expansion .my requirement is to prepare pilot project in small scale DIY-BIO lab which can further be scaled.
if possible provide me vectors, cell line and seedstock. i am new to DIY-BIO hence i need helping hands of veterans like you ppl .. your help be highly appreciated
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