Hello,
Il giorno lunedì 2 marzo 2015 00:49:33 UTC+1, Nathan McCorkle ha scritto:
-- thank you for your help. I read the documents, but, as on every document I can find on the web, there are only normalized values of intensity.
I know that if I put together SYBR Green and DNA the fluorescence increase by 1000 times, but how much light does it emits respectively to the one that hit the molecole?
I mean that I want to understand if I can measure that fluorescence with a sensor that for example has this curve of sensitivity:
http://s24.postimg.org/bjc1ifhyd/Capture.png
So I would to know the order of magnitude of this fluorescence (that obviously is related to the intensity of light with which I hit the SYBR Green).
Thanks and regards,
Gabriele
Il giorno lunedì 2 marzo 2015 00:49:33 UTC+1, Nathan McCorkle ha scritto:
This should be helpful:
http://www.theinstituteoffluorescence. com/Publication%202/JoFL% 20paper.pdf
I searched google scholar with these keywords:
'sybr chemistry fluorophore chromophore'
That link was the 4th result.
On Sun, Mar 1, 2015 at 8:20 AM, Gabriele Borelli <borel...@gmail.com> wrote:
> Hello,
> I am studying Electronic Bioengineering at University of Bologna. I am
> beginning to study the working principle of qPCR, more specific I am now
> focusing on SYBRGreen. I would like to do some optical tests on that
> chemical compound. My biological knowledges are not excellent, so I thought
> that my first step could be finding a datasheet of this subastance. I did
> several researches on google, but I can't find any documents where the
> behavior of the SYBRGreen is completely described. I would like to know:
> - emitted light / incident light
> - wavelenght of the emitted light
> - sensibility / wavelength of the incident light
> - changes with temperature (?)
> So I would like to have a datasheet, something that describes SYBRGreen
> entirely.
> Where can I find this document?
>
> Thanks and regards,
> Gabriele
>
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