Hello,
Il giorno domenica 1 marzo 2015 22:35:31 UTC+1, Gabriele Borelli ha scritto:
-- thank you for your help. I read the documents, but, as on every document I can find on the web, there are only normalized values of intensity.
I know that if I put together SYBR Green and DNA the fluorescence increase by 1000 times, but how much light does it emits respectively to the one that hit the molecole?
I mean that I want to understand if I can measure that fluorescence with a sensor that for example has this curve of sensitivity:
[url=http://postimg.org/image/kr49z4p0h/][img]http://s24.postimg.org/kr49z4p0h/Capture.jpg[/img][/url]
So I would to know the order of magnitude of this fluorescence (that obviously is related to the intensity of light with which I hit the SYBR Green).
Thanks and regards,
Gabriele
Il giorno domenica 1 marzo 2015 22:35:31 UTC+1, Gabriele Borelli ha scritto:
Hello,I am studying Electronic Bioengineering at University of Bologna. I am beginning to study the working principle of qPCR, more specific I am now focusing on SYBRGreen. I would like to do some optical tests on that chemical compound. My biological knowledges are not excellent, so I thought that my first step could be finding a datasheet of this subastance. I did several researches on google, but I can't find any documents where the behavior of the SYBRGreen is completely described. I would like to know:- emitted light / incident light- wavelenght of the emitted light- sensibility / wavelength of the incident light- changes with temperature (?)So I would like to have a datasheet, something that describes SYBRGreen entirely.Where can I find this document?Thanks and regards,Gabriele
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