I'll place it in a plasmid and transform Salmonella with it. If plasmid will be too large - I'll use electroporation. And, as I mentioned before - it is not pathogenic to humans.
Am Dienstag, 26. Mai 2015 19:13:40 UTC+3 schrieb Nathan McCorkle:
-- Am Dienstag, 26. Mai 2015 19:13:40 UTC+3 schrieb Nathan McCorkle:
On Mon, May 25, 2015 at 10:56 PM, Dorif <dor...@gmail.com> wrote:
> In my country it is not the easiest way, because at us PCR is not well
> developed.(
In that case, what will you do with the operon once you get it? Try to
induce horizontal gene transfer? I wouldn't try that with potentially
pathogenic samples... get some kind of stripped-down plasmid with
otherwise non-scary genes on it... you don't want dead/half-dead rats
to become a carrier and spreading genes that aren't helpful for
humans.
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