Re: [DIYbio] Re: Anybody has E.coli lactose operon in a plasmid?

Yes in regular e coli lac Opteron is there intact. In cloning strains there is typically a deletion of the region containing lac operon and a lac Z (omega, I.e. alpha deleted) YA cassette added back in lambda prophage.
>matt

Nathan McCorkle <nmz787@gmail.com> wrote:


On Tue, May 26, 2015 at 9:24 AM, Matt Lawes <matt@insysx.com> wrote:
> From a technical angle, the lactose operon (lacZYA) is rather large. Cloning
> plasmids with blue /white screens use a fragment (alpha) of lacZ and the
> rest on a propagate (lambda).

(reading it with prophage)

I thought the rest was intact in the E.coli genome, with just lacZ
being knocked-out.

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