Re: [DIYbio] Re: Where to buy nerve tissue?

I wonder is C. elegans would work.. it has nerves... ?

On Tue, Jan 26, 2016 at 6:21 PM, Nathan McCorkle <nmz787@gmail.com> wrote:
> It might be interesting to compare the proteome and transcriptome of
> the bug grown by itself (if it can survive without nerves), and then
> grown co-cultured. Then again with bugs post-infiltration of the nerve
> culture.
>
> SIMpore sends samples, if the pore size is too big (big enough for the
> bugs to get through to the nerve culture side) then they can also
> supply windows with smaller holes, but you'll need to setup your own
> culture environment (you can get away with molding some (relatively
> pure, as in PDMS, though hardware-store grade natural/clear might also
> work, though I expect there might be conditioners that would leak
> diffuse into the culture medium) silicone with bits of removable
> plastic in a glass petri dish, probably).
>
> On Tue, Jan 26, 2016 at 4:11 PM, coolcash2004 <davidconwayj@gmail.com> wrote:
>> Thanks for the info and reply. It is suspected that persister Borrelia
>> burrows deeply into nerve tissue - so I'm not sure if using cell lines would
>> reflect the environment as well. I'm beginning to think that I might have
>> to do this without the nerve tissue first due to cost. Other than buying
>> the actual tissue, which I can afford, would there be any other major costs
>> with doing the culture on tissue vs in a medium? I understand that
>> contamination and failure to culture could pose the biggest risks?
>>
>> Thanks again,
>>
>>
>> On Tuesday, January 26, 2016 at 10:20:10 AM UTC-5, Reshma Bhatnagar wrote:
>>>
>>> Here is a great review on choosing an appropriate strategy for
>>> co-culturing your cells. Nathan's suggestion of using CytoVu is excellent if
>>> you can afford it.
>>>
>>> If you'll be co-culturing, my suggestion on animal brains is mostly out,
>>> as they will only act as a nutrient medium, and won't help much with your
>>> work if you intend to study cell-cell interaction/work on proteomics in the
>>> context of nervous tissue. The alternative is you sit with a butcher as they
>>> do their business -- not a particularly enticing prospect. Even if you do go
>>> ahead with the latter, keep in mind that culturing non-foetal neural cells
>>> are notoriously difficult, though you may have more luck with glia. Here is
>>> a protocol on culturing neural cells but I have never tested it and can't
>>> vouch for it. Here is a protocol for Schwann cells (haven't worked with them
>>> either).
>>>
>>> Human peripheral nerve tissue will be pretty hard (if not nearly
>>> impossible) to find. You'll get cell lines easily enough (recommended). They
>>> will be expensive, though. Here are some links:
>>>
>>> ThermoFisher
>>> SigmaAldrich
>>>
>>> All of these considerations will affect your model.
>>>
>>> Do note that you'll need to maintain sterile conditions etc. Contamination
>>> will wreak havoc with your experiment.
>>>
>>>
>>> On Tuesday, January 26, 2016 at 5:58:32 AM UTC+5:30, coolcash2004 wrote:
>>>>
>>>> I'm trying to culture persister borrelia on peripheral nerve tissue so
>>>> that I can submit the bacteria cells for proteomics analysis.
>>>>
>>>> On Monday, January 25, 2016 at 8:48:22 AM UTC-5, Reshma Bhatnagar wrote:
>>>>>
>>>>> "I'm trying to adapt an existing procedure for culturing bacteria to
>>>>> instead culture the bacteria on nerve tissue"
>>>>>
>>>>> Why are you trying to grow bacteria on nerve tissue? Seems wasteful and
>>>>> expensive. If culturing bacteria is your concern, there are several media
>>>>> that can be used and are widely available. At the end of the day, the
>>>>> nutritional requirements of the bacteria need to be met in order to culture
>>>>> them, and I don't see any special reason to use nerve tissue to culture any
>>>>> bacteria.
>>>>>
>>>>> What is the goal for culturing your bacteria?
>>>>>
>>>>> If you are attempting to do so to explore treatments of bacterial
>>>>> infections that affect the brain by culturing bacteria on nerve tissue and
>>>>> using drugs to kill the bacteria, the attempt would be meaningless because:
>>>>>
>>>>> 1. Both need to pass the blood brain barrier in living organisms
>>>>> 2. Antibiotics have been well studied anyway
>>>>> 3. Organisms may be resistant to the antibiotics you test
>>>>>
>>>>> If you want to go ahead anyway, I suggest buying offal/animal brains
>>>>> from a butcher. I will however, reiterate that it seems pointless. Perhaps,
>>>>> if you could outline what you propose to do, I can come up with a more
>>>>> helpful reply.
>>>>>
>>>>> On Monday, January 25, 2016 at 6:11:57 AM UTC+5:30, coolcash2004 wrote:
>>>>>>
>>>>>> Anyone have experience working with nerve tissue? Where can you buy
>>>>>> it? Any major challenges I should be aware of? I'm trying to adapt an
>>>>>> existing procedure for culturing bacteria to instead culture the bacteria on
>>>>>> nerve tissue. Thanks again,
>>
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>
>
>
> --
> -Nathan



--
-Nathan

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