Re: [DIYbio] Re: Where to buy nerve tissue?

It might be interesting to compare the proteome and transcriptome of
the bug grown by itself (if it can survive without nerves), and then
grown co-cultured. Then again with bugs post-infiltration of the nerve
culture.

SIMpore sends samples, if the pore size is too big (big enough for the
bugs to get through to the nerve culture side) then they can also
supply windows with smaller holes, but you'll need to setup your own
culture environment (you can get away with molding some (relatively
pure, as in PDMS, though hardware-store grade natural/clear might also
work, though I expect there might be conditioners that would leak
diffuse into the culture medium) silicone with bits of removable
plastic in a glass petri dish, probably).

On Tue, Jan 26, 2016 at 4:11 PM, coolcash2004 <davidconwayj@gmail.com> wrote:
> Thanks for the info and reply. It is suspected that persister Borrelia
> burrows deeply into nerve tissue - so I'm not sure if using cell lines would
> reflect the environment as well. I'm beginning to think that I might have
> to do this without the nerve tissue first due to cost. Other than buying
> the actual tissue, which I can afford, would there be any other major costs
> with doing the culture on tissue vs in a medium? I understand that
> contamination and failure to culture could pose the biggest risks?
>
> Thanks again,
>
>
> On Tuesday, January 26, 2016 at 10:20:10 AM UTC-5, Reshma Bhatnagar wrote:
>>
>> Here is a great review on choosing an appropriate strategy for
>> co-culturing your cells. Nathan's suggestion of using CytoVu is excellent if
>> you can afford it.
>>
>> If you'll be co-culturing, my suggestion on animal brains is mostly out,
>> as they will only act as a nutrient medium, and won't help much with your
>> work if you intend to study cell-cell interaction/work on proteomics in the
>> context of nervous tissue. The alternative is you sit with a butcher as they
>> do their business -- not a particularly enticing prospect. Even if you do go
>> ahead with the latter, keep in mind that culturing non-foetal neural cells
>> are notoriously difficult, though you may have more luck with glia. Here is
>> a protocol on culturing neural cells but I have never tested it and can't
>> vouch for it. Here is a protocol for Schwann cells (haven't worked with them
>> either).
>>
>> Human peripheral nerve tissue will be pretty hard (if not nearly
>> impossible) to find. You'll get cell lines easily enough (recommended). They
>> will be expensive, though. Here are some links:
>>
>> ThermoFisher
>> SigmaAldrich
>>
>> All of these considerations will affect your model.
>>
>> Do note that you'll need to maintain sterile conditions etc. Contamination
>> will wreak havoc with your experiment.
>>
>>
>> On Tuesday, January 26, 2016 at 5:58:32 AM UTC+5:30, coolcash2004 wrote:
>>>
>>> I'm trying to culture persister borrelia on peripheral nerve tissue so
>>> that I can submit the bacteria cells for proteomics analysis.
>>>
>>> On Monday, January 25, 2016 at 8:48:22 AM UTC-5, Reshma Bhatnagar wrote:
>>>>
>>>> "I'm trying to adapt an existing procedure for culturing bacteria to
>>>> instead culture the bacteria on nerve tissue"
>>>>
>>>> Why are you trying to grow bacteria on nerve tissue? Seems wasteful and
>>>> expensive. If culturing bacteria is your concern, there are several media
>>>> that can be used and are widely available. At the end of the day, the
>>>> nutritional requirements of the bacteria need to be met in order to culture
>>>> them, and I don't see any special reason to use nerve tissue to culture any
>>>> bacteria.
>>>>
>>>> What is the goal for culturing your bacteria?
>>>>
>>>> If you are attempting to do so to explore treatments of bacterial
>>>> infections that affect the brain by culturing bacteria on nerve tissue and
>>>> using drugs to kill the bacteria, the attempt would be meaningless because:
>>>>
>>>> 1. Both need to pass the blood brain barrier in living organisms
>>>> 2. Antibiotics have been well studied anyway
>>>> 3. Organisms may be resistant to the antibiotics you test
>>>>
>>>> If you want to go ahead anyway, I suggest buying offal/animal brains
>>>> from a butcher. I will however, reiterate that it seems pointless. Perhaps,
>>>> if you could outline what you propose to do, I can come up with a more
>>>> helpful reply.
>>>>
>>>> On Monday, January 25, 2016 at 6:11:57 AM UTC+5:30, coolcash2004 wrote:
>>>>>
>>>>> Anyone have experience working with nerve tissue? Where can you buy
>>>>> it? Any major challenges I should be aware of? I'm trying to adapt an
>>>>> existing procedure for culturing bacteria to instead culture the bacteria on
>>>>> nerve tissue. Thanks again,
>
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-Nathan

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