[DIYbio] Re: Using poxviruses as base for vector construction

Very interesting. Does anyone know the regulations of working with them? Are they biodsafety S1? Or S3? I mean if you use your brain, it will tell you that there is absolutely no harm in a virus that has been completely depleted in its weapons. But regulatory bodies may tend to differ - the label Pox may sound scary. 

On Friday, September 23, 2016 at 9:19:44 PM UTC+2, Dorif wrote:

They can replicate, but they cause disease only in immunodeficient animals. So, yes, they do not contain immune-system evading proteins.

Am Freitag, 23. September 2016 22:17:41 UTC+3 schrieb Mega [Andreas Stuermer]:

I dimmly remember reading about vaccine trials where they put HIV proteins into a common vaccinia virus to elicit antibody response. Google should be able to find it-  

So the ones you have are disarmed and replication deficient? Or replicating and just deleted in all immune-system evading proteins? 

On Friday, September 23, 2016 at 9:03:19 PM UTC+2, Dorif wrote:

What do you think about this? On the one side they have huge genome, what makes possible to insert large sequences. On the other side they replicate outside of nucleus, so they can be used only for something like CRISPR/Cas systems which guidance RNA and proteins can penetrate nucleus. Of course I do not propose to use Variola major virus. I think something like fowlpox or myxoma viruses will be good for this. And currently I have both myxoma (strain V-82) and fowlpox (strain K) viruses, both vaccinal ones.

Thanks for advice!