Yes, one detail: fowlpox virus can not replicate in mammalian cells, myxoma virus can replicate in rabbit cells or in some of the human tumor cells (it is now researched as perspective oncolytic virus). If we want to create vector capable to replicate in human cells we must replace some of the replication-related genes of these viruses with Variola, Vaccinia or Cowpox ones. Or simply use Vaccinia or Cowpox as basis.
Am Freitag, 23. September 2016 22:03:19 UTC+3 schrieb Dorif:
-- Am Freitag, 23. September 2016 22:03:19 UTC+3 schrieb Dorif:
What do you think about this? On the one side they have huge genome, what makes possible to insert large sequences. On the other side they replicate outside of nucleus, so they can be used only for something like CRISPR/Cas systems which guidance RNA and proteins can penetrate nucleus. Of course I do not propose to use Variola major virus. I think something like fowlpox or myxoma viruses will be good for this. And currently I have both myxoma (strain V-82) and fowlpox (strain K) viruses, both vaccinal ones.Thanks for advice!
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