Think outside the box! Long before the advent of DNA Sequencers we use to run our own Sanger sequencing gels. Basic gel electrophoresis at it's best! The multi-lane gels were polyacrylamide and either 36 inches or 48 inches long for resolution. It sucked when the gel leaked before setting! Back then the reactions were labeled with P32 or S35 but I'm certain the modern fluorescent DNA dyes would work since you have one terminator per lane - yes, 4 lanes per sequence run! A single run would give us ~200-250bp of sequence (on a good day!) but the modern PCR-based reactions would improve that.
-- Mind you the cost of a commercial sequence run would likely be be less but this would be a fun DIYbio project.
Cheers,
Scott
http://www.opensciencenet.org
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