Hi Aubrey,
-- Interesting silk project. For your question have a look at this abstract.
https://www.ncbi.nlm.nih.gov/pubmed/20819369
When making media we normally autoclave (~120oC@~20minutes) the yeast extract (and the other components). Try boiling your media for awhile if you don't have a pressure cooker.
Let us know how your project goes.
Cheers,
Scott
http://www.opensciencenet.org
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