Science

• We participated in bio.academany.org 's HTGAA program. It has been amazing, and exhausting, and I'm sad that the 2016 class is almost over.
  
• We hosted OpenBio Codefest 2016. The most focused and productive group of developers I've ever run into.
  
• Some of our members attended HOPEXI where the bio presence has been growing nicely. I'm ooking forward to 2018!
  
• We participated in CGSR's Independent Biotechnology:Innovation-regulation workshop.  It was a great event, and I hope that the conversation continues.
• Some of our members attended Biohack The Planet, which was fantastic, and I hope that it becomes a regular event. (Ideally one that is scheduled to proceed or follow SynbiobetaSF just a *little* more closely in the future.)
• For me, the best part was interacting with other spaces. We got a ton of help and support from the folks at BioCurious, CCL, Genspace, and the-odin, and a bunch of great advice from Sebastian.  This is a wonderful community, and we are truly grateful to be able to be a part of it.

• Synthetic Bio presence at SXSW
• SynbiobetaSF (This is the first year I had to miss it)
• BioHacker village at DefCon

OH YEAH! How could I miss that one? I heard that was a good time, and will be watching to get it in my calendar for next year. :)

On Saturday, December 31, 2016 at 1:48:23 PM UTC-5, Koeng wrote:

BioHack The Planet conference was this year as well, which was a great event for meeting other people in the community

On Saturday, December 31, 2016 at 7:54:05 AM UTC-8, Kevin Chen wrote:

Hi all!

It's that time of the year again! Anyone have reflections for the past year? Big wins for DIYBio in 2016? Excited for something coming up next year?

Here are a few of my highlights off the top of my head:

1. At Bricobio we finally found some physical space to start a lab (woo! hometeam)
2. BentoLab ran their kickstarter campaign
   
3. Canada held its first DIYbio Summit, bringing together all the Canadian DIYbio spaces, which is a challenge since we're so spread out.
4. I visited the Waag Society and the L4B (The LAB? / LA DIYbio) for the first time. Great spaces and amazing people!
5. In hacker conferences in the US: HOPEXI happened again featuring more bio content and a biohacking village, DefCon also had a biohacking village. Shout outs to the organizers!
6. Thanks to the ODIN a lot of us got to do a DIY CRISPR experiment, which is just one of the many amazing things coming from those guys.

I'm sure that I'm missing many other cool things that happened. Anyone else?

Hi all!

It's that time of the year again! Anyone have reflections for the past year? Big wins for DIYBio in 2016? Excited for something coming up next year?

Here are a few of my highlights off the top of my head:

1. At Bricobio we finally found some physical space to start a lab (woo! hometeam)
2. BentoLab ran their kickstarter campaign
   
3. Canada held its first DIYbio Summit, bringing together all the Canadian DIYbio spaces, which is a challenge since we're so spread out.
4. I visited the Waag Society and the L4B (The LAB? / LA DIYbio) for the first time. Great spaces and amazing people!
5. In hacker conferences in the US: HOPEXI happened again featuring more bio content and a biohacking village, DefCon also had a biohacking village. Shout outs to the organizers!
6. Thanks to the ODIN a lot of us got to do a DIY CRISPR experiment, which is just one of the many amazing things coming from those guys.

I'm sure that I'm missing many other cool things that happened. Anyone else?

1. At Bricobio we finally found some physical space to start a lab (woo! hometeam)
2. BentoLab ran their kickstarter campaign
   
3. Canada held its first DIYbio Summit, bringing together all the Canadian DIYbio spaces, which is a challenge since we're so spread out.
4. I visited the Waag Society and the L4B (The LAB? / LA DIYbio) for the first time. Great spaces and amazing people!
5. In hacker conferences in the US: HOPEXI happened again featuring more bio content and a biohacking village, DefCon also had a biohacking village. Shout outs to the organizers!
6. Thanks to the ODIN a lot of us got to do a DIY CRISPR experiment, which is just one of the many amazing things coming from those guys.

Hey All,

I've been lurking quietly on the mailing list for the past 4 years.

I'm looking for full stack software freelance jobs. After 2 years in the cloud computing industry, I left my previous (all-remote) Python job in Cisco in August 18, 2016 in search of a team making tangible products.  My experience has been in APIs, web applications in Django, Flask, rest client libraries, single-page dashboards, and command line tools. I've worked at Rotten Tomatoes (1 year), Piston (1 year) and then at Cisco (1 year) after Piston got acquired in June 2015. Open source projects that I've volunteered on as a technical contributor in the past include openhatch.org, django 1.7, 18F projects.

I'm in the San Francisco Bay Area, I can work remotely. If you have a project in mind, let me know and we can discuss. I prefer to program primarily in Python, my favorite programming language. 

Happy Holidays,

Susan Tan

Do you have a primer for it? 

On Dec 29, 2016, at 9:33 AM, hlalibe . <lalibe...@gmail.com> wrote:

i asked them to sequence only one gene, and they indeed specify SAnger sequencing in their quote.

On 29 December 2016 at 18:15, Abizar Lakdawalla <abi...@gmail.com> wrote:

Two kinds. If you are sequencing a gene of less than 1000 bp then you would use Sanger sequencing which is read by capillary electrophoresis. That costs about $10. If you are sequencing the whole Genome of E.coli, 5,000,000 bp, then that would cost $100's.

On Dec 29, 2016 8:51 AM, "Hugues" <lalibe...@gmail.com> wrote:

Hello,

10 to 20 usd ??

mmm....

Here is a quote i received , for 760.80 usd for 2 samples, shipping charges excluded

Are we talking about the same work here ? Sorry i'm a newbie to this.

Can you give me the name of a company doing this work for that kind of price ? I will contact them.

***************************

Our Offer

1. DNA isolation

DNA isolation (column) 2 - 160.40 320.80

Total 320.80

- DNA will be isolated from your samples using an appropriate kit. 

2. Primer design & synthesis and PCR testing and optimization

Primer design including synthesis 2- 25.00 50.00

PCR set-up and optimization 1 300.00 300.00

Total 350.00

3. PCR clean-up and Sanger sequencing

Single PCR purification, price per sample  2- 9.00  18.00

Economy Run 4- 18.00  72.00

Total 90.00

- PCR products will be purified and bi-directionally Sanger sequenced. The results of the Sanger

sequencing will be sent to the customer for further analysis.

Total costs for the characterization: CHF 760.80

*****************

On Saturday, 24 December 2016 00:44:52 UTC+1, Mega [Andreas Stuermer] wrote:

Basically any synthesis company will sell you primers ("oligos"). And they will also do a sequencing run. Make sure to inquire about prizing first. Total costs should be 10 to 20$ plus shipment of your DNA to them.



On Tuesday, December 20, 2016 at 6:24:33 PM UTC+1, Hugues wrote:

Hi guys,

I have 2 samples of E. Coli for which i would like to have a specific gene sequenced,

where is the cheapest place i can get that done ?

I checked online, there are many, not sure where to start , any recommendations ?

i live in Switzerland

thanks

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diy...@googlegroups.com. To unsubscribe from this group, send email to diybio+un...@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+un...@googlegroups.com.
To post to this group, send email to diy...@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/f7eb41eb-15df-46c4-b3c0-b680b16e6daa%40googlegroups.com.
For more options, visit https://groups.google.com/d/optout.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diy...@googlegroups.com. To unsubscribe from this group, send email to diybio+un...@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to a topic in the Google Groups "DIYbio" group.
To unsubscribe from this topic, visit https://groups.google.com/d/topic/diybio/cB-6U66xvgM/unsubscribe.
To unsubscribe from this group and all its topics, send an email to diybio+un...@googlegroups.com.
To post to this group, send email to diy...@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CAND8PcObUscEOsaF3eCjPoboArNtwVu_a6%3D5D-4TXaFCQXB%2B3A%40mail.gmail.com.
For more options, visit https://groups.google.com/d/optout.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diy...@googlegroups.com. To unsubscribe from this group, send email to diybio+un...@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+un...@googlegroups.com.
To post to this group, send email to diy...@googlegroups.com.
Visit this group at https://groups.google.com/group/diybio.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CAKqKegY-uuBwJSNHLjTkDH076Tu9HMp%3D2bqLU1YFkyY8cGbHDw%40mail.gmail.com.
For more options, visit https://groups.google.com/d/optout.