Re: [DIYbio] Re: Where can i get an E. Coli gene sequenced for cheap ?

Sounds very expensive for what it is.
Depending on what you currently have access to you can extract the DNA yourself and create a PCR product which you can then clean up and send off for sequencing along with the primers you used to amplify it.

If I'm amplifying something out of E.coli I tend to just do a boiling lysis (colony in a PCR tube with H2O and 10-15 mins at 95C +) then use a couple of microlitres of the supernatant of that in a PCR reaction as template.
Seems hugely overkill that they are offering a full on premium extraction just for that (sales rep bumping up the invoice maybe?)...

Design primers as Roninlaw mentions above. I tend to order mine from Eurofins Genomics / MWG (whatever they're called these days) they're based in southern germany but the DHL or FedEx things to me in England within a couple of days so fairly handy.
I normally expect spend 2 or 3 GBP per primer.

The PCR product/reaction can then be cleaned up using a kit like https://www.promega.co.uk/products/dna-purification-quantitation/dna-fragment-purification/wizard-sv-gel-and-pcr-clean_up-system/   (that's the one I use, there is a method for this process in sambrooks/maniartis but it's more trouble than it's worth vs just paying for a kit, that one is 75GBP list price for 50 preps so that works out to about £1.50 per prep).

Then send that cleaned up reaction and some of each primer as per the sequencing company's requirements. One run of sanger sequencing tends to cost anything like 5 - 10 GBP. The pricing tends to get a bit better if you pay for several in advance.

So I reckon you could at least knock a zero off the end of the price their offering if you're equipped and willing to do some basic molecular biology. :)

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