Re: [DIYbio] Re: Where can i get an E. Coli gene sequenced for cheap ?

no i don't have a primer, they need to provide

it's included in the quote i showed in my earlier post

On Thursday, 29 December 2016 18:37:48 UTC+1, Roninlaw wrote: 

Do you have a primer for it? 

On Dec 29, 2016, at 9:33 AM, hlalibe . <lalibe...@gmail.com> wrote:

i asked them to sequence only one gene, and they indeed specify SAnger sequencing in their quote.

On 29 December 2016 at 18:15, Abizar Lakdawalla <abi...@gmail.com> wrote:

Two kinds. If you are sequencing a gene of less than 1000 bp then you would use Sanger sequencing which is read by capillary electrophoresis. That costs about $10. If you are sequencing the whole Genome of E.coli, 5,000,000 bp, then that would cost $100's.

On Dec 29, 2016 8:51 AM, "Hugues" <lalibe...@gmail.com> wrote:

Hello,

10 to 20 usd ??

mmm....

Here is a quote i received , for 760.80 usd for 2 samples, shipping charges excluded

Are we talking about the same work here ? Sorry i'm a newbie to this.

Can you give me the name of a company doing this work for that kind of price ? I will contact them.

***************************

Our Offer

1. DNA isolation

DNA isolation (column) 2 - 160.40 320.80

Total 320.80

- DNA will be isolated from your samples using an appropriate kit. 

2. Primer design & synthesis and PCR testing and optimization

Primer design including synthesis 2- 25.00 50.00

PCR set-up and optimization 1 300.00 300.00

Total 350.00

3. PCR clean-up and Sanger sequencing

Single PCR purification, price per sample  2- 9.00  18.00

Economy Run 4- 18.00  72.00

Total 90.00

- PCR products will be purified and bi-directionally Sanger sequenced. The results of the Sanger

sequencing will be sent to the customer for further analysis.

Total costs for the characterization: CHF 760.80

*****************

On Saturday, 24 December 2016 00:44:52 UTC+1, Mega [Andreas Stuermer] wrote:

Basically any synthesis company will sell you primers ("oligos"). And they will also do a sequencing run. Make sure to inquire about prizing first. Total costs should be 10 to 20$ plus shipment of your DNA to them.



On Tuesday, December 20, 2016 at 6:24:33 PM UTC+1, Hugues wrote:

Hi guys,

I have 2 samples of E. Coli for which i would like to have a specific gene sequenced,

where is the cheapest place i can get that done ?

I checked online, there are many, not sure where to start , any recommendations ?

i live in Switzerland

thanks

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