You can use NaCl to final conc of 0.5M and add EtOH to a final concentration of 70-80%. If you put it in the -20 C freezer for a few hours, it will precipitate easily. There are also methods with isopropanol (rubbing alcohol), which only needs
35-40%, but salts are less soluble so don't put it in the freezer, just chill to 4 C.
-- 35-40%, but salts are less soluble so don't put it in the freezer, just chill to 4 C.
On Thursday, May 9, 2019 at 10:13:30 PM UTC+8, Andreas "Mega" Stuermer wrote:
Hi!I am looking on ethanol precipitation protocols... But they all use NaOAc (which I don't have). Some indicate that you might use NaCl as well (which I have on stock). Does anyone have a working protocol how to do an ethanol precipitation with NaCl?
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