DNA precipitation is a fairly forgiving process.
a) Add 1/10 volume saturated NaCl (5M or stronger), then 2 volumes EtOH.
b) Precipitation works OK at room temperature, but if the amount of DNA is ng, then chilling to -20C helps.
c) Spin down, wash pellet w/ 70% EtOH to remove salt.
Cheers,
Jim Lund
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