I think I read in a paper, long ago, that LPS contamination might contribute to DNA vaccine immunogenicity.
On Saturday, March 7, 2020 at 3:21:32 AM UTC+1, Michael Flynn wrote:
-- I would assume that expressing the protein in coli is not ideal - you get so little protein vs so much LPS. It might just cause the innate immune system to go bonker. Our immune system is trained to respond to the tiniest ammounts of LPS. Also you will get a B-cell response this way, but not much T-cell response. De-novo synthesis of proteins and display of fragments thereof mediates ADCC activity and the lack thereof is (for example) the reason why Herpes vaccines have failed so far.
I'm not often one to say this isn't DIY-ble but considering everything the danger seems very high compared to potential gains. Sure, given enough training you could turn your basement into a certified 50k$ lab that is suitable. May be easier to join a lab.
As far as I can tell, live recombinant or live attenuated viruses are the new thing for effective vaccines - here they inserted an Ebola protein into a "harmless-to-human" virus that replicates https://www.nejm.org/doi/full/10.1056/NEJMoa1414216
But then, there's a lot of regulation around that topic and it is not smart to play around with this in a DIY setting.
Sometimes there's a reason why things are mostly done in academia.
On Saturday, March 7, 2020 at 3:21:32 AM UTC+1, Michael Flynn wrote:
Often in vaccine development, in addition to target antigens that you want to raise antibodies against, an adjuvant, something that stimulates the immune system like lipopolysaccharide, must be added in order to generate an adaptive immune response against the antigen. E.coli is covered with lipopolysaccharide, which reacts strongly to complement (antibacterial factors in blood), and so would be a good candidate for an adjuvant. This line of thinking leads to an "easy vaccine" where you express the antigen in E.coli and thermally lyse the cells, dilute them to some concentration in water for injection, and there you have your vaccine. In an outbreak like coronavirus, making vaccines like this would seem to be "low risk, high reward" in that nothing bad seems likely to come of it, but you could potentially immunize against this bad outbreak.Anyone have any actual literature on this?
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