Re: [DIYbio] Re: DIY COVID-19 PCR test

Thank you for the response Michael.  I had deleted my post because I hadn't searched for "Ct value" properly (was the top hit for "RT PCR Ct value")

I don't have any firsthand knowledge of the challenges associated with "working towards [clinical] implementation solutions" so I can't comment on this.

Hopefully myself or those close won't be infected (if there are statistically we look safe) but if so then trying to replicate that same paper might prove interesting / be useful.  There will unfortunately be differences though as I don't have access to a RT-PCR.  Instead I'll look for the appropriate sized bands on a gel.

Kind regards,

AJP

On Friday, March 6, 2020 at 4:48:23 PM UTC, Michael Crone wrote:

Ct value is the number of cycles before you get above a certain threshold fluorescence. Lower means more sensitive in the context of the paper.

There is a massive issue with everyone developing their own tests because it just causes massive fragmentation. Ideally researchers should be working towards implementation solutions. Developing tests is easy, actually implementing them in a clinical context is the difficult part.

Michael

On Fri, 6 Mar 2020 at 16:42, AJP <bio...@gmail.com> wrote:

Derek had replied here linking to: https://www.molecularcloud.org/How-to-detect-the-2019-novel-coronavirus.html

On a separate note does anyone know what "Ct value" means in the context of this paper please?

Results and Discussion
Validation of qRT-PCR assay
The Ct value was not produced from negative control, indicating the reaction was done aseptically.

Thank you,

AJP

 

On Wednesday, March 4, 2020 at 5:51:25 PM UTC, AJP wrote:

Hi Derek,

What company is that?  And how much / what's their lead time?  I'm asking as some of us from the London BioHackSpace want to be able to test ourselves and those near to us... to minimise us infecting others if / when we're infected.

Thank you,

AJP

p.s. if you're interested you can join the slack group and join the #lets-beat-corona channel , though we'll be sure to post here with any relevant info / decisions / purchases.

On Friday, February 28, 2020 at 5:19:20 PM UTC, Derek wrote:

Hey Tito,

Been doing some research on this. With the long incubation time I'd like to know when it's circulating in the community before cases are seen. Have found an rt-pcr protocol and located a company that will sell me a plasmid with part of the capsid envelope to use as a positive control. Biobasic has all the primers cheaply. Still working on a collection protocol to allow me to swab banisters, etc. A bunch of folks at the victoria makerspace are interested in helping so will eventually be documenting our steps. Happy to share any details.

Derek

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